The Relationship between Serum Zonulin and Innate Immunity in Patients with Inflammatory Bowel Disease

Author:

Khusainova Gusel12ORCID,Genkel Vadim3ORCID,Kuznetsova Alla1,Nikushkina Karina4,Saenko Anna1ORCID,Abramovskikh Olga5,Dolgushina Anastasiya12

Affiliation:

1. Department of Hospital Therapy, South-Ural State Medical University, Vorovskogo St. 64, 454092 Chelyabinsk, Russia

2. Chelyabinsk Regional Clinical Hospital, Vorovskogo St. 70, 454048 Chelyabinsk, Russia

3. Department of Internal Medicine, South-Ural State Medical University, Vorovskogo St. 64, 454092 Chelyabinsk, Russia

4. Research Institute of Immunology, South-Ural State Medical University, Vorovskogo St. 64, 454092 Chelyabinsk, Russia

5. Department of Clinical Laboratory Diagnostics, South-Ural State Medical University, Vorovskogo St. 64, 454092 Chelyabinsk, Russia

Abstract

The aim of the study was to investigate the relationship between the serum zonulin and the cellular immunity in patients with ulcerative colitis (UC) and Crohn’s disease (CD). The study included 97 patients, 13 (13.4%) patients with CD and 84 (86.6%) patients with UC. The concentration of zonulin in the serum was studied using the ELISA kits. The investigation of the circulated leukocyte subpopulation was carried out through flow cytometry. The functional activity of the circulating neutrophils was studied using the absorption capacity of the monodisperse polystyrene latex particles and indicators of oxygen-dependent metabolism in the nitroblue tetrazolium test. The serum zonulin concentration in CD patients was significantly higher compared with UC patients (p = 0.003). The zonulin concentration directly correlated with the functional activity of the circulating neutrophils. Patients with a zonulin concentration > 472.4 pg/mL had a significantly higher number of band neutrophils (p = 0.0104), CD3+CD8+ cells (p = 0.0212), NK cells (p = 0.0161), and lower–CD19+ cells (p = 0.0034). Among the IBD patients, zonulin was associated with IBD severity. An increase in the serum concentration of zonulin was associated with an increase in the functional activity of circulating neutrophils and an increase in the number of CD3+CD8+ cells, NK cells, and a decrease in the number of CD19+ cells.

Publisher

MDPI AG

Reference28 articles.

1. Inflammatory Bowel Disease Collaborators (2020). The global, regional, and national burden of inflammatory bowel disease in 195 countries and territories, 1990–2017: A systematic analysis for the Global Burden of Disease Study 2017. Lancet Gastroenterol. Hepatol., 5, 17.

2. Worldwide incidence and prevalence of inflammatory bowel disease in the 21st century: A systematic review of population-based studies;Ng;Lancet,2017

3. Zonulin is a marker of epithelial and endothelial barrier functions in non-communicable diseases (narrative review);Zybina;Russ. J. Evid.-Based Gastroenterol.,2022

4. Zonulin and its regulation of intestinal barrier function: The biological door to inflammation, autoimmunity, and cancer;Fasano;Physiol. Rev.,2011

5. All disease begins in the (leaky) gut: Role of zonulin-mediated gut permeability in the pathogenesis of some chronic inflammatory diseases;Fasano;F1000Research,2020

Cited by 2 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3