Epistatic Relationship between MGV1 and TRI6 in the Regulation of Biosynthetic Gene Clusters in Fusarium graminearum

Author:

Shostak Kristina1,González-Peña Fundora Dianevys23ORCID,Blackman Christopher14ORCID,Witte Tom15ORCID,Sproule Amanda1,Overy David1ORCID,Eranthodi Anas2,Thakor Nehal3ORCID,Foroud Nora A.2ORCID,Subramaniam Rajagopal14ORCID

Affiliation:

1. Ottawa Research and Development Centre, Agriculture and Agri-Food Canada, Ottawa, ON K1A 0C6, Canada

2. Lethbridge Research and Development Centre, Agriculture and Agri-Food Canada, Lethbridge, AB T1J 4B1, Canada

3. Department of Chemistry and Biochemistry, University of Lethbridge, Lethbridge, AB T1K 4M4, Canada

4. Department of Cell and System Biology, University of Toronto, Toronto, ON M5S 3B2, Canada

5. Department of Biology, University of Ottawa, Ottawa, ON K1N 6N5, Canada

Abstract

Genetic studies have shown that the MAP kinase MGV1 and the transcriptional regulator TRI6 regulate many of the same biosynthetic gene clusters (BGCs) in Fusarium graminearum. This study sought to investigate the relationship between MGV1 and TRI6 in the regulatory hierarchy. Transgenic F. graminearum strains constitutively expressing MGV1 and TRI6 were generated to address both independent and epistatic regulation of BGCs by MGV1 and TRI6. We performed a comparative transcriptome analysis between axenic cultures grown in nutrient-rich and secondary metabolite-inducing conditions. The results indicated that BGCs regulated independently by Mgv1 included genes of BGC52, whereas genes uniquely regulated by TRI6 included the gene cluster (BGC49) that produces gramillin. To understand the epistatic relationship between MGV1 and TRI6, CRISPR/Cas9 was used to insert a constitutive promoter to drive TRI6 expression in the Δmgv1 strain. The results indicate that BGCs that produce deoxynivalenol and fusaoctaxin are co-regulated, with TRI6 being partially regulated by MGV1. Overall, the findings from this study indicate that MGV1 provides an articulation point to differentially regulate various BGCs. Moreover, TRI6, embedded in one of the BGCs provides specificity to regulate the expression of the genes in the BGC.

Funder

Agriculture and Agri-Food Canada

Alberta Innovates–Technology Futures graduate student scholarships

Publisher

MDPI AG

Subject

Plant Science,Ecology, Evolution, Behavior and Systematics,Microbiology (medical)

Reference29 articles.

1. Involvement of the osmosensor histidine kinase and osmotic stress-activated protein kinases in the regulation of secondary metabolism in Fusarium graminearum;Ochiai;Biochem. Biophys. Res. Commun.,2007

2. The ATF/CREB transcription factor Atf1 is essential for full virulence, deoxynivalenol production, and stress tolerance in the cereal pathogen Fusarium graminearum;Bormann;Mol. Plant-Microbe Interact.,2013

3. The TOR signaling pathway regulates vegetative development and virulence in Fusarium graminearum;Yu;New Phytol.,2014

4. Functional analysis of the Fusarium graminearum phosphatome;Yun;New Phytol.,2015

5. TRI6 and TRI10 play different roles in the regulation of deoxynivalenol (DON) production by cAMP signalling in Fusarium graminearum;Jiang;Environ. Microbiol.,2016

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