Asymmetry Index Evaluation of Cerebral Volume and Cerebral Blood Flow in Neonatal Hypoxic–Ischemic Encephalopathy

Abstract

The aim of the present study was to longitudinally evaluate the differences in cerebral volume and cerebral blood flow (CBF) on the right and left sides in rats with neonatal hypoxic–ischemic encephalopathy (HIE) using magnetic resonance imaging and the Rice–Vannucci model. Unilateral ligation of the left common carotid artery was performed on 8-day-old rats, followed by mild (1 h, n = 6) or severe (2 h, n = 7) hypoxic exposure. T2-weighted (T2W) and CBF images were obtained at 1 h and 1, 3, and 7 days following the HI insult. The cerebral volume (Vlesion and Vcontrol), CBF in both hemispheres (lesion and control sides), and asymmetry indices of the cerebral volume (AIvolume) and CBF (AICBF) were calculated for each group. Slight hyperintensities were noted in the lesion-side hemispheres on T2W images at 1 h and 1 day in both groups, as were pronounced hyperintensities at days 3 and 7 in the severe group. AIvolume was positive (Vlesion > Vcontrol) in the mild and severe groups until days 1 and 3, respectively, and changed to negative on days 3 and 7 in the mild and severe groups. These results suggest that the prolonged positive AIvolume prior to day 3 in the severe group was caused by long-term cell swelling following severe HI insult.