A pH Monitoring Algorithm for Orifice Plate Culture Medium

Abstract

Recently, there has been renewed interest in cell therapy, which plays a key role in the clinical research of genetic diseases, advanced blood disease, and other diseases. It shows considerable clinical application value and is known as “the new pillar of future medicine”. Automatic cell culture and operation technology is the key to ensuring scale, standardization, and stability between batches of therapeutic cells. The pH of the cell culture medium is vital for cell growth. Most cells are suitable for growth at pH 7.2~7.4. A pH of cell culture medium lower than 6.8 or higher than 7.6 is harmful to cells, and cells will degenerate or even die. At present, the monitoring method of cell culture medium pH of automatic cell culture equipment is mainly a visual observation method, which can not accurately or quickly reflect changes in the cell culture medium. To address the issue of monitoring of cell culture fluid pH for automated cell culture equipment and the inability to employ invasive sensors to measure pH during well plate culture, a pH monitoring method for orifice plate culture medium algorithm based on HSV (hue, saturation, value) model is proposed by studying the changes of cell culture medium in the process of cell culture. The research presented here reveals the laws of cell culture fluid pH change and its color moment, and the intelligent monitoring of cell culture fluid pH was successfully achieved. The problem of non-destructive monitoring of the pH of cell culture fluids in well plates is also addressed.