A Mathematical Modeling and Statistical Analysis of Phycobiliprotein Fluorescence Decay under Exposure to Excitation Light

Abstract

Photosynthetic phycobiliprotein complexes from Spirulina maxima were purified and fractioned by gel chromatography. A mathematical model was developed for the fractionated phycobiliprotein complexes to successfully represent the fluorescence decay rate under exposure to excitation light. Each fractionated complex had a different ratio of phycobiliproteins, such as allophycocyanin, phycocyanin, or phycoerythrin, but their fluorescence decay trends were determined to statistically have a high similarity. The mathematical model was derived based on mass balance in the sense that the fluorescence of phycobiliprotein complex was linearly dependent on its mass. The model considered both exponentially decreasing (early light-exposure period) and linearly decreasing (later period), and successfully fit the whole period of fluorescence decay trend.