Steam Explosion Pretreatment of Beechwood. Part 2: Quantification of Cellulase Inhibitors and Their Effect on Avicel Hydrolysis

Abstract

Biomass pretreatment is a mandatory step for the biochemical conversion of lignocellulose to chemicals. During pretreatment, soluble compounds are released into the prehydrolyzate that inhibit the enzymatic hydrolysis step. In this work, we investigated how the reaction conditions in steam explosion pretreatment of beechwood (severity: 3.0–5.25; temperature: 160–230 °C) influence the resulting amounts of different inhibitors. Furthermore, we quantified the extent of enzyme inhibition during enzymatic hydrolysis of Avicel in the presence of the prehydrolyzates. The amounts of phenolics, HMF, acetic acid and formic acid increased with increasing pretreatment severities and maximal quantities of 21.6, 8.3, 43.7 and 10.9 mg/gbeechwood, respectively, were measured at the highest severity. In contrast, the furfural concentration peaked at a temperature of 200 °C and a severity of 4.75. The presence of the prehydrolyzates in enzymatic hydrolysis of Avicel lowered the glucose yields by 5–26%. Mainly, the amount of phenolics and xylose and xylooligomers contributed to the reduced yield. As the maximal amounts of these two inhibitors can be found at different conditions, a wide range of pretreatment severities led to severely inhibiting prehydrolyzates. This study may provide guidelines when choosing optimal pretreatment conditions for whole slurry enzymatic hydrolysis.