Affiliation:
1. Department of Large Animal Diseases with Clinic, Institute of Veterinary Medicine, WULS—SGGW (Warsaw University of Life Sciences, Szkoła Główna Gospodarstwa Wiejskiego), Nowoursynowska 100, 02-797 Warsaw, Poland
Abstract
The lack of fertilization and early pregnancy loss is seen in the quality and maturation of the oocytes. The environment of the first divisions and maturation of the oogonia, which is also a reflection of the quality of the oocyte, is the follicular fluid (FF). Thus, the purpose of this study was to investigate the variations in parameters such as pH, pCO2, pO2, standard HCO3−, actual HCO3−, base excess (BE), base excess of extracellular fluid (BE ecf), ctCO2, ions: Na+, K+, actual ionized calcium Ca2+, adjusted ionized calcium (at pH 7.4) Ca2+ (7.4), Cl−, anion gap (AnGap), and glucose in FF collected from different size follicles in dairy cattle. The most apparent differences were related to pH, K+, and Ca2+ 7.4 concentration in comparison to changes in follicle size (p < 0.05). Several trends were also evaluated as an increase in follicular size was followed by an increase in pH, BE, and Ca2+ 7.4 value and a decrease in the K+ concentration (p < 0.05). In conclusion, there are changes in FF formularies associated with the size of follicles. However, more research is necessary to establish the reference value, which then could be one of the factors describing the quality of the follicle and the developmental potential of the associated oocyte.
Subject
General Veterinary,Animal Science and Zoology
Reference36 articles.
1. (2023, March 23). The Embassy of the Netherlands in Poland Quick Scan Polish Dairy Sector Contents. Available online: https://www.agroberichtenbuitenland.nl/actueel/nieuws/2020/10/21/quick-scan-polish-dairy-sector.
2. The Estrus Detection Problem: New Concepts, Technologies, and Possibilities;Senger;J. Dairy Sci.,1994
3. 2020 Statistics of embryo production and transfer in domestic farm animals;Joao;Embryo Technol. Newsl.,2021
4. Developmental Potential in Bovine Oocytes Is Related to Cumulus-Oocyte Complex Grade, Calcium Current Activity, and Calcium Stores;Edwards;Reproduction,1974
5. Metabolite and ionic composition of follicular fluid from different-sized follicles and their relationship to serum concentrations in dairy cows;Leroy;Anim. Reprod. Sci.,2004