Study on Influencing Factors of Nicotinamide Transdermal Absorption In Vitro and the Establishment of an Evaluation Method

Abstract

The goal of this research was to study the factors influencing the in vitro transdermal penetration of nicotinamide and to establish an evaluation method for the in vitro transdermal absorption of nicotinamide. The permeability of nicotinamide was investigated with Franz diffusion cell in vitro transcutaneous assays, and the effect of the receiving solution composition, receiving solution pH, skin type, diffusion cell temperature, active ingredient concentration, supply quantity, and product dosage form on its permeation was investigated separately by high-performance liquid chromatography. The best assay for the transdermal absorption of nicotinamide was established—there was a better transdermal absorption performance, more stable system, better applicability, and better reproducibility when the receiving solution was PBS (phosphate-buffered saline) solution, the pH was 7.4, the membrane was pig ear skin, the temperature was 37 °C, the concentration of nicotinamide was 3%, and the dose of the test substance was 2 g. In the three cosmetic dosage forms of toning lotion, milk lotion, and gel, the permeability of milk lotion was the highest, followed by toning lotion and gel.