Identification and Characterization of circRNAs under Drought Stress in Moso Bamboo (Phyllostachys edulis)

Abstract

Circular RNAs (circRNAs) are a class of endogenous noncoding RNAs formed by 3′-5′ ligation during splicing. They play an important role in the regulation of transcription and miRNA in eukaryotes. Drought is one of the detrimental abiotic stresses that limit plant growth and productivity. How circRNAs influence the response to drought stress in moso bamboo (Phyllostachys edulis) remains elusive. In this study, we investigate the expression pattern of circRNAs in moso bamboo at 6 h, 12 h, 24 h and 48 h after drought treatment by deep sequencing and bioinformatics analysis and identify 4931 circRNAs, 52 of which are differentially expressed (DEcircRNAs) in drought-treated and untreated moso bamboo. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses of the host genes that generate the DEcircRNAs indcate that these DEcircRNAs are predicted to be involved in biochemical processes in response to drought, such as ubiquitin-mediated proteolysis, calcium-dependent protein kinase phosphorylation, amino acid biosynthesis and plant hormone signal transduction including abscisic acid. In addition, some circRNAs are shown to act as sponges for 291 miRNAs. Taken together, our results characterize the transcriptome profiles of circRNAs in drought responses and provide new insights into resistance breeding of moso bamboo.