Metagenomics Analysis of Breast Microbiome Highlights the Abundance of Rothia Genus in Tumor Tissues

Author:

Kartti Souad12,Bendani Houda12,Boumajdi Nasma12,Bouricha El Mehdi12ORCID,Zarrik Oumaima1,EL Agouri Hajar3,Fokar Mohamed4ORCID,Aghlallou Youssef5ORCID,EL Jaoudi Rachid126,Belyamani Lahcen236ORCID,Elkhannoussi Basma3,Ibrahimi Azeddine127

Affiliation:

1. Biotechnology Lab (MedBiotech), Bioinova Research Center, Rabat Medical & Pharmacy School, Mohammed V University in Rabat, Rabat 10100, Morocco

2. Mohammed VI Center for Research & Innovation (CM6), Rabat 10000, Morocco

3. Pathology Department, Oncology National Institute, Rabat Medical and Pharmacy School, Mohammed Vth University in Rabat, Rabat 10100, Morocco

4. Center for Biotechnology and Genomics, Texas Tech University, Lubbock, TX 79409, USA

5. Institute of Cancer Research, Fez 30000, Morocco

6. Emergency Department, Military Hospital Mohammed V, Rabat 10000, Morocco

7. Mohammed VI University of Health Sciences, Casablanca 20000, Morocco

Abstract

Breast cancer is one of the main global priorities in terms of public health. It remains the most frequent cancer in women and is the leading cause of their death. The human microbiome plays various roles in maintaining health by ensuring a dynamic balance with the host or in the appearance of various pathologies including breast cancer. In this study, we performed an analysis of bacterial signature differences between tumor and adjacent tissues of breast cancer patients in Morocco. Using 16S rRNA gene sequencing, we observed that adjacent tissue contained a much higher percentage of the Gammaproteobacteria class (35.7%) while tumor tissue was characterized by a higher percentage of Bacilli and Actinobacteria classes, with about 18.8% and 17.2% average abundance, respectively. Analysis of tumor subtype revealed enrichment of genus Sphingomonodas in TNBC while Sphingomonodas was predominant in HER2. The LEfSe and the genus level heatmap analysis revealed a higher abundance of the Rothia genus in tumor tissues. The identified microbial communities can therefore serve as potential biomarkers for prognosis and diagnosis, while also helping to develop new strategies for the treatment of breast cancer patients.

Funder

Institute of Cancer Research, Morocco

Publisher

MDPI AG

Subject

Medicine (miscellaneous)

Cited by 8 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Guideline for designing microbiome studies in neoplastic diseases;GeroScience;2024-06-26

2. Pre-Examination of Breast Cancer Dataset Using Exploratory Data Analysis (EDA) Approach;2024 International Conference on Computational Intelligence and Computing Applications (ICCICA);2024-05-23

3. Breast Cancer Exposomics;Life;2024-03-18

4. A journey from omics to clinicomics in solid cancers: Success stories and challenges;Advances in Protein Chemistry and Structural Biology;2024

5. The Breast Microbiome in Breast Cancer Risk and Progression: A Narrative Review;Cancer Epidemiology, Biomarkers & Prevention;2023-11-09

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