The Effect of Dentin Contamination by Topical Anesthetics on Micro-Shear Bond Strength: An In Vitro Experiment

Abstract

Topical anesthetics are commonly used to minimize pain and anxiety during dental procedures. Research is scarce on the influence of topical anesthetics on bond strength. Thus, this research evaluated the effect of dentin contamination by topical anesthetic solution and gel on the micro-shear bond strengths of etch-and-rinse and self-etch bonding systems. Ninety transversally hemi-sectioned dentin discs were prepared and randomly assigned to three groups: no contamination (control group), contamination with topical anesthetic solution (Xylonor spray, Septodont), and contamination with topical anesthetic gel (Xylonor gel, Septodont). Each contamination group was subdivided into two subgroups (n = 15) based on whether the adhesive system was etch-and-rinse (Optibond Solo Plus, Kerr) or self-etch (Optibond XTR, Kerr). Tygon tubes with resin composite (Filtek Z350 XT, 3M ESPE) were placed on each surface and light cured. After 24 h, a universal testing machine was used to measure micro-shear bond strength (MPa). Furthermore, nine additional specimens of non-contaminated and contaminated dentin were prepared and scanned by a scanning electron microscope. The data of micro-shear bond strength were analyzed using two-way ANOVA, and narrative analysis was used to qualitatively interpret visual data of the micro-morphology of dentin from the scanning electron microscope. No significant differences in micro-shear bond strength among different contamination groups and adhesive systems were found (p > 0.05). The results are supported by micro-morphology of the treated dentin surfaces and modes of failure, as the micro-morphology was similar among contamination and control groups. There was no significant impact of topical anesthetic forms and dentin bonding systems on the micro-shear bond strength, which was supported by the micro-morphology from a scanning electron microscope.