Study of Canine Distemper Virus Presence in Catalonia’s Wild Carnivores through H Gene Amplification and Sequencing

Author:

Huang Junhao1ORCID,Cortey Martí1ORCID,Darwich Laila1ORCID,Griffin Jenna1ORCID,Obón Elena2ORCID,Molina Rafael2ORCID,Martín Margarita1ORCID

Affiliation:

1. Department of Animal Health and Anatomy, Autonomous University of Barcelona (UAB), 08193 Cerdanyola del Vallès, Spain

2. Torreferrussa Wildlife Rehabilitation Centre, Catalan Wildlife Service-Forestal Catalana S.A., 08130 Santa Perpètua de Mogoda, Spain

Abstract

Canine distemper virus (CDV) is recognised worldwide as an important pathogen in both domestic and wild carnivores. Few data are available on its impact and spread on the wildlife/wildlife–domestic animal–environment interface. This study, aimed at developing a conservation-oriented control strategy, analysed 89 sick or deceased animals from 2019 to 2023 at the Wildlife Rehabilitation Centre in Torreferrussa. RT-PCR and sequencing of the partial H gene were used to detect and analyse CDV in tissues. The total positive percentage was 20.22% (18/89), comprising 13 red foxes (44.8%), 4 European badgers (28.6%), and 1 American mink (4.5%), while 24 Eurasian otters tested negative. Phylogenetic analysis indicated that all of the CDV strains belong to the European lineage. Geographically distant individuals and different species shared the same viral strain, suggesting a strong capacity of CDV for interspecies and long-distance transmission. This calls for further research, particularly focusing on potential impacts of CDV on endangered carnivores.

Publisher

MDPI AG

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