Affiliation:
1. Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou 225009, China
2. International Joint Research Laboratory in Universities of Jiangsu Province of China for Domestic Animal Germplasm Resources and Genetic Improvement, Yangzhou University, Yangzhou 225009, China
3. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
4. International Centre for Agricultural Research in the Dry Areas, Addis Ababa 999047, Ethiopia
5. CSIRO Agriculture and Food, 306 Carmody Rd, St Lucia, QLD 4067, Australia
Abstract
Previous studies have demonstrated that CUX1 could contribute to the proliferation of DPCs in vitro, but the upstream transcriptional regulatory mechanisms of CUX1 remain largely unknown. This study aimed to investigate the upstream transcriptional regulators of CUX1 to enhance our comprehension of the mechanism of action of the CUX1 gene in ovine DPCs. Initially, the JASPAR (2024) software was used to predict the upstream target transcription factors for the CUX1 gene. Subsequently, through RT-qPCR and a double luciferase reporter assay, the interaction between SP1, KROX20, and CUX1 was established, respectively. The results indicated that SP1 and KROX20 were two highly reliable upstream transcription regulators for the CUX1 gene. Additionally, we found that SP1 promoted the proliferation of DPCs by overexpressing SP1 in DPCs, and KROX20 inhibited the proliferation of DPCs by overexpressing KROX20 in DPCs. These findings are also consistent with the transcriptional regulation of CUX1 by SP1 and KROX20, respectively. This study suggests that the effect of DPC proliferation in vitro by CUX1 may regulated by the transcription factors SP1 and KROX20.
Funder
National Natural Science Foundation of China
Natural Science Foundation of Jiangsu Province
Natural Science Foundation of the Jiangsu Higher Education Institutions of China
Major New Varieties of Agricultural Projects in Jiangsu Province
National Natural Science Foundation of China-CGIAR
Major Project of the Natural Science Foundation of Xinjiang Uyghur Autonomous Region
High-end Foreign Expert Introduction Project
Jiangsu 333 Distinguished Talents Project Foundation
Science and Technology Plan Project of Yangzhou
Distinguished Talents Project Foundation of Yangzhou University
Open Project Program of Joint International Research Laboratory of Agriculture and Agri-Product Safety
Ministry Education of China, Yangzhou University