Identification of Potential miRNA-mRNA Regulatory Network Associated with Regulating Immunity and Metabolism in Pigs Induced by ASFV Infection
Author:
Pang Zhongbao1, Chen Shiyu1, Cui Shuai2, Zhai Wenzhu1, Huang Ying1, Gao Xintao3, Wang Yang1, Jiang Fei4, Guo Xiaoyu1, Hao Yuxin4, Li Wencai4, Wang Lei4, Zhu Hongfei1, Wu Jiajun4, Jia Hong1
Affiliation:
1. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China 2. College of Animal Medicine, Shandong Vocational Animal Science and Veterinary College, Weifang 261061, China 3. Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China 4. China Animal Disease Control Center, Beijing 100026, China
Abstract
African swine fever (ASF) is a devastating infectious disease in domestic pigs caused by African swine fever virus (ASFV) with a mortality rate of about 100%. However, the understanding of the interaction between ASFV and host is still not clear. In this study, the expression differences and functional analysis of microRNA (miRNA) in porcine peripheral blood lymphocytes of ASFV infected pigs and healthy pigs were compared based on Illumina high-throughput sequencing, then the GO and KEGG signal pathways were analyzed. The miRNA related to immunity and inflammation were screened, and the regulatory network of miRNA-mRNA was drawn. A total of 70 differentially expressed miRNAs were found (p ≤ 0.05). Of these, 45 were upregulated and 25 were downregulated in ASFV-infected pigs vs. healthy pigs. A total of 8179 mRNA genes targeted by these 70 differentially expressed miRNA were predicted, of which 1447 mRNA genes were targeted by ssc-miR-2320-5p. Five differentially expressed miRNA were validated by RT-qPCR, which were consistent with the RNA-Seq results. The GO analysis revealed that a total of 30 gene functions were significantly enriched, including 7 molecular functions (MF), 13 cellular components (CC), and 10 biological processes (BP). The KEGG enrichment analysis revealed that the differentially expressed genes were significantly enriched in pathways related to immunity, inflammation, and various metabolic processes, in which a total of two downregulated miRNAs after infection and eight upregulated miRNAs related to immunity and inflammation were screened in ASFV-infected pigs vs. healthy pigs. The network of miRNA-mRNA showed that the mRNA target genes were strongly regulated by ssc-miR-214, ssc-miR-199b-3p, and ssc-miR-199a-3p. The mRNA target genes were enriched into the MAPK signaling pathway, Toll-like receptor signaling pathway, TNF signaling pathway, and IL-17 signaling pathway by using a KEGG enrichment analysis. Therefore, ASFV could regulate immunity and metabolism-related pathways in infected pigs by inducing differential expression of miRNAs. These results provided a new basis for further elucidating the interactions between ASFV and the host as well as the immunity regulation mechanisms of ASFV, which will be conducive to better controlling ASF.
Funder
National Key Research and Development Program of China the Yunnan Key Research and Development project the earmarked fund for CARS the Agricultural Science and Technology Innovation Program
Subject
General Veterinary,Animal Science and Zoology
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