A Route to the Colorimetric Detection of Alpha-Fetoprotein Based on a Smartphone

Author:

Liu Junjie1,Geng Qingfubo1,Geng Zhaoxin12ORCID

Affiliation:

1. School of Information Engineering, Minzu University of China, Beijing 100081, China

2. Key Laboratory of Ethnic Language Intelligent Analysis and Security Governance of MOE, Minzu University of China, Beijing 100081, China

Abstract

Alpha-fetoprotein (AFP) is a key marker for early cancer detection and assessment. However, the current detection methods struggle to balance accuracy with the need for decentralized medical treatment. To address this issue, a new AFP analysis platform utilizing digital image colorimetry has been developed. Functionalized gold nanoparticles act as colorimetric agents, changing from purple-red to light gray-blue when exposed to different AFP concentrations. A smartphone app captures these color changes and calculates the AFP concentration in the sample. To improve detection accuracy, a hardware device ensures uniform illumination. Testing has confirmed that this system can quantitatively analyze AFP using colorimetry. The limit of detection reached 0.083 ng/mL, and the average accuracy reached 90.81%. This innovative method enhances AFP testing by offering portability, precision, and low cost, making it particularly suitable for resource-limited areas.

Funder

National Key Research and Development Plan of China

National Natural Science Foundation of China

Beijing Natural Science Foundation

Application of Minzu University of China

GC Project Business Plan of Minzu University of China

Publisher

MDPI AG

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