Author:
Galay Remil L.,Talactac Melbourne R.,Ambita-Salem Bea V.,Chu Dawn Maureen M.,Costa Lali Marie O. dela,Salangsang Cinnamon Mae A.,Caracas Darwin Kyle B.,Generoso Florante H.,Babelonia Jonathan A.,Vergano Joeneil L.,Berana Lena C.,Sandalo Kristina Andrea C.,Divina Billy P.,Alvarez Cherry R.,Mago Emmanuel R.,Andoh Masako,Tanaka Tetsuya
Abstract
Rickettsia and Coxiella burnetii are zoonotic, tick-borne pathogens that can cause febrile illnesses with or without other symptoms in humans, but may cause subclinical infections in animals. There are only a few reports on the occurrence of these pathogens in cattle and water buffalo in Southeast Asia, including the Philippines. In this study, molecular detection of Rickettsia and C. burnetii in the blood and in the Rhipicephalus (Boophilus) microplus ticks of cattle and water buffalo from five provinces in Luzon Island of the Philippines was done. A total of 620 blood samples of cattle and water buffalo and 206 tick samples were collected and subjected to DNA extraction. After successful amplification of control genes, nested PCR was performed to detect gltA of Rickettsia and com1 of C. burnetii. No samples were positive for Rickettsia, while 10 (cattle = 7, water buffaloes = 3), or 1.6% of blood, and five, or 1.8% of tick samples, were C. burnetii-positive. Sequence analysis of the positive amplicons showed 99–100% similarity to reported C. burnetii isolates. This molecular evidence on the occurrence of C. burnetii in Philippine ruminants and cattle ticks and its zoonotic nature should prompt further investigation and surveillance to facilitate its effective control.
Funder
Japan Society for the Promotion of Science
University of the Philippines
Subject
Infectious Diseases,Public Health, Environmental and Occupational Health,General Immunology and Microbiology
Reference64 articles.
1. Genetic diversity and relationship of domestic buffalo and cattle breeds and crossbreeds (subfamily Bovinae) in the Philippines based on the cytochrome c oxidase I (COI) gene sequence;Bondoc;Philipp. Agric. Sci.,2013
2. Current Knowledge on Phylogeny and Taxonomy of Rickettsia spp.
3. Q fever
Cited by
14 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献