Acute Phase Proteins in Dogs with Natural Infection by Trypanosoma cruzi
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Published:2023-05-31
Issue:6
Volume:8
Page:299
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ISSN:2414-6366
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Container-title:Tropical Medicine and Infectious Disease
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language:en
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Short-container-title:TropicalMed
Author:
Rivadeneira-Barreiro Pilar12ORCID, Montes-de-Oca-Jiménez Roberto1ORCID, Zambrano-Rodríguez Pablo2ORCID, Vázquez-Chagoyán Juan Carlos1, Gutiérrez-Castillo Adriana del Carmen1, Pardo-Marin Luis3ORCID, Franco-Martínez Lorena3ORCID, Cerón José Joaquín3, Martínez-Subiela Silvia3ORCID
Affiliation:
1. Centro de Investigación y Estudios Avanzados en Salud Animal, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Km 15.5 Carretera Panamericana Toluca-Atlacomulco, Toluca 50200, Mexico 2. Departamento de Veterinaria, Facultad de Ciencias Veterinarias, Universidad Técnica de Manabí, Portoviejo 130105, Ecuador 3. Interdisciplinary Laboratory of Clinical Analysis, Interlab-UMU, Regional Campus of International Excellence ‘Campus Mare Nostrum’, University of Murcia, 30100 Murcia, Spain
Abstract
Acute phase proteins have been used as tools for the diagnosis, monitoring, and prognosis of several diseases in domestic animals. However, the dynamics of these proteins in infection by Trypanosoma cruzi, the causative agent of Chagas disease in dogs, is still unknown. The aim of this study was to determine concentrations of acute phase proteins (C-reactive protein, haptoglobin, ferritin and paraoxonase-1) in dogs in a coastal town of Ecuador, with natural Trypanosoma cruzi infection with or without seroreactivity of Ehrlichia canis, Ehrlichia ewingii, Anaplasma phagocytophilum, Anaplasma platys, Borrelia burgdorferi and Dirofilaria immitis. For the detection of Trypanosoma cruzi serum antibodies, two different antigen-based enzyme-linked immunosorbent assay tests were implemented. For the detection of seroreactivity of Ehrlichia canis, Ehrlichia ewingii, Anaplasma phagocytophilum, Anaplasma platys, Borrelia burgdorferi and Dirofilaria immitis, an IDEXX SNAP® 4Dx® test was used. To determine the concentration of C-reactive protein and ferritin, an immunoturbidimetric assay was used; haptoglobin concentration was measured using a commercial colorimetric method validated in dogs; a spectrophotometric method was used to determine the serum concentration of paraoxonase-1. Results showed a reduction in the serum levels of paraoxonase-1 in Trypanosoma cruzi-seroreactive dogs, either with or without seroreactivity to other vector-borne diseases. A serum ferritin increment was observed in Trypanosoma cruzi-seroreactive dogs with seroreactivity to any other vector-borne diseases. Our findings suggest that paraoxonase-1 levels are reduced in Trypanosoma cruzi-seroreactive dogs without evident clinical signs of Chagas disease, despite their seroreactivity to the other vector-borne diseases studied. These findings could indicate an oxidative stress response in Trypanosoma cruzi-seroreactive dogs with no evident signs of inflammation.
Funder
Universidad Autónoma del Estado de México
Subject
Infectious Diseases,Public Health, Environmental and Occupational Health,General Immunology and Microbiology
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