Assessment of the Risk of Exotic Zika Virus Strain Transmission by Aedes aegypti and Culex quinquefasciatus from Senegal Compared to a Native Strain-Reference-Cited by-同舟云学术

Assessment of the Risk of Exotic Zika Virus Strain Transmission by Aedes aegypti and Culex quinquefasciatus from Senegal Compared to a Native Strain

Published:2023-02-20 Issue:2 Volume:8 Page:130
ISSN:2414-6366
Container-title:Tropical Medicine and Infectious Disease
language:en
Short-container-title:TropicalMed

Author:

Gaye Alioune¹,Fall Cheikh²³^ORCID,Faye Oumar³^ORCID,Dupont-Rouzeyrol Myrielle⁴^ORCID,Ndiaye El Hadji¹^ORCID,Diallo Diawo¹^ORCID,de Andrade Zanotto Paolo Marinho⁵^ORCID,Dia Ibrahima¹^ORCID,Weaver Scott C.⁶^ORCID,Diallo Mawlouth¹

Affiliation:

1. Pole de Zoologie Médicale, Institut Pasteur de Dakar, 36, Avenue Pasteur, Dakar BP 220, Senegal

2. Pole de Microbiologie, Institut Pasteur de Dakar, 36, Avenue Pasteur, Dakar BP 220, Senegal

3. Pole de Virologie, Institut Pasteur de Dakar, 36, Avenue Pasteur, Dakar BP 220, Senegal

4. URE Dengue et Arboviroses, Institut Pasteur de Nouvelle-Calédonie, Réseau International des Instituts Pasteur, BP 61, CEDEX, 98845 Noumea, New Caledonia

5. Microbiology Department, Institute of Biomedical Sciences, University of São Paulo (USP), São Paulo 05508020, SP, Brazil

6. World Reference Center for Emerging Viruses and Arboviruses, Institute for Human Infections and Immunity, Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA

Abstract

Zika virus (ZIKV) shows an enigmatic epidemiological profile in Africa. Despite its frequent detection in mosquitoes, few human cases have been reported. This could be due to the low infectious potential or low virulence of African ZIKV lineages. This study sought to assess the susceptibility of A. aegypti and C. quinquefasciatus to ZIKV strains from Senegal, Brazil, and New Caledonia. Vertical transmission was also investigated. Whole bodies, legs/wings and saliva samples were tested for ZIKV by real-time PCR to estimate infection, dissemination and transmission rates as well as the infection rate in the progeny of infected female A. aegypti. For A. aegypti, the Senegalese strain showed at 15 days post-exposure (dpe) a significantly higher infection rate (52.43%) than the Brazilian (10%) and New Caledonian (0%) strains. The Brazilian and Senegalese strains were disseminated but not detected in saliva. No A. aegypti offspring from females infected with Senegalese and Brazilian ZIKV strains tested positive. No infection was recorded for C. quinquefasciatus. We observed the incompetence of Senegalese A. aegypti to transmit ZIKV and the C. quinquefasciatus were completely refractory. The effect of freezing ZIKV had no significant impact on the vector competence of Aedes aegypti from Senegal, and vertical transmission was not reported in this study.

Funder

ACIP

European Union

NIH/NIAID

Publisher

MDPI AG

Subject

Infectious Diseases,Public Health, Environmental and Occupational Health,General Immunology and Microbiology

Link

https://www.mdpi.com/2414-6366/8/2/130/pdf

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