Exploring Reactive Oxygen Species Accumulation in Allium fistulosum L. Seeds Exposed to Different Storage Conditions-Reference-Cited by-同舟云学术

Exploring Reactive Oxygen Species Accumulation in Allium fistulosum L. Seeds Exposed to Different Storage Conditions

Published:2024-02-09 Issue:1 Volume:3 Page:123-132
ISSN:2674-1024
Container-title:Seeds
language:en
Short-container-title:Seeds

Author:

Padula Gregorio¹^ORCID,Macovei Anca²^ORCID,Ravasio Adriano³,Pagano Andrea²,Jr Dueñas Conrado²,Xia Xianzong¹^ORCID,Hołubowicz Roman¹^ORCID,Balestrazzi Alma²^ORCID

Affiliation:

1. Department of Plant Pathology, Seed Science and Technology, Faculty of Agriculture, Horticulture and Bioengineering, Poznan University of Life Sciences, 60-637 Poznan, Poland

2. Department of Biology and Biotechnology ‘L. Spallanzani’, University of Pavia, Via Ferrata 1, 27100 Pavia, Italy

3. NeoruraleHub, Innovation Center Giulio Natta, Località Cascina Darsena, Giussago, 27010 Pavia, Italy

Abstract

The purpose of this work was to investigate the production of reactive oxygen species (ROS) in Allium fistulosum seeds stored under different conditions. Optimized seed storage conditions are essential to maintain seed viability, otherwise accumulation of ROS-induced oxidative damage can lead to seed aging. The A. fistulosum seed lots used in this study have been selected based on their breeding background and reproduction site. Seed samples were stored up to 22 months under six different conditions of temperature (25, 10, and 7.5 °C) and relative humidity (RH) (25% and 45% RH). A germination test and ROS quantification assay were performed on the samples collected after 12 and 22 months of storage, respectively. Within a time-window of 10 months, the tested seed lots evidenced a decrease in the germination rate associated with increased ROS levels. Correlation analysis also showed that ROS production was influenced by genotype. The reported data showed that ROS accumulation was dependent on the storage condition and genotype. Some of the tested seed lots appeared to be prone to ROS accumulation, independent of storage conditions. On the other hand, specific condition storages (25 °C, 25% RH; 25 °C, 45% RH; 10 °C, 25% RH; 7.5 °C, 25% RH) resulted in a lower impact on seed aging.

Funder

University of Pavia Crowdfunding

Publisher

MDPI AG

Link

https://www.mdpi.com/2674-1024/3/1/10/pdf

Reference21 articles.

1. Plants of the genus Allium as antibacterial agents: From tradition to pharmacy;Mnayer;Cell. Mol. Biol.,2016

2. Allium vegetables: Traditional uses, phytoconstituents, and beneficial effects in inflammation and cancer;Alam;Crit. Rev. Food Sci. Nutr.,2022

3. FAO (2006). Seed and Seed Quality: Technical Information for FAO Emergency Staff, FAO Seed and Plant Genetic Resources Service.

4. Loss of vigour and viability in aged onion (Allium cepa L.) seeds;Khan;Int. J. Agr. Biol.,2004

5. Pagano, A., Macovei, A., Xia, X., Padula, G., Hołubowicz, R., and Balestrazzi, A. (2023). Seed priming applied to onion-like crops: State of the art and open questions. Agronomy, 13.