Celecoxib, a Non-Steroidal Anti-Inflammatory Drug, Exerts a Toxic Effect on Human Melanoma Cells Grown as 2D and 3D Cell Cultures
Author:
Venuta Alessandro1, Nasso Rosarita2, Gisonna Armando3, Iuliano Roberta3, Montesarchio Sara3, Acampora Vittoria1, Sepe Leandra3ORCID, Avagliano Angelica1, Arcone Rosaria2, Arcucci Alessandro1, Ruocco Maria Rosaria3
Affiliation:
1. Department of Public Health, University of Naples Federico II, 80131 Naples, Italy 2. Department of Movement Sciences and Wellness, University of Naples “Parthenope”, 80133 Naples, Italy 3. Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, 80131 Naples, Italy
Abstract
Cutaneous melanoma (CM) remains one of the leading causes of tumor mortality due to its high metastatic spread. CM growth is influenced by inflammation regulated by prostaglandins (PGs) whose synthesis is catalyzed by cyclooxygenases (COXs). COX inhibitors, including non-steroidal anti-inflammatory drugs (NSAIDs), can inhibit tumor development and growth. In particular, in vitro experiments have shown that celecoxib, a NSAID, inhibits the growth of some tumor cell lines. However, two-dimensional (2D) cell cultures, used in traditional in vitro anticancer assays, often show poor efficacy due to a lack of an in vivo like cellular environment. Three-dimensional (3D) cell cultures, such as spheroids, are better models because they can mimic the common features displayed by human solid tumors. Hence, in this study, we evaluated the anti-neoplastic potential of celecoxib, in both 2D and 3D cell cultures of A2058 and SAN melanoma cell lines. In particular, celecoxib reduced the cell viability and migratory capability and triggered the apoptosis of melanoma cells grown as 2D cultures. When celecoxib was tested on 3D melanoma cell cultures, the drug exerted an inhibitory effect on cell outgrowth from spheroids and reduced the invasiveness of melanoma cell spheroids into the hydrogel matrix. This work suggests that celecoxib could represent a new potential therapeutic approach in melanoma therapy.
Funder
Regione Campania “SATIN”
Subject
Paleontology,Space and Planetary Science,General Biochemistry, Genetics and Molecular Biology,Ecology, Evolution, Behavior and Systematics
Reference60 articles.
1. Cutaneous Melanoma: From Pathogenesis to Therapy (Review);Leonardi;Int. J. Oncol.,2018 2. Combining Molecular and Immunohistochemical Analyses of Key Drivers in Primary Melanomas: Interplay between Germline and Somatic Variations;Bruno;Oncotarget,2018 3. Differential Responsiveness to BRAF Inhibitors of Melanoma Cell Lines BRAF V600E-Mutated;Sastry;J. Transl. Med.,2020 4. Solar Ultraviolet-Induced DNA Damage Response: Melanocytes Story in Transformation to Environmental Melanomagenesis;Sarkar;Env. Mol. Mutagen.,2020 5. Millán-Esteban, D., Peña-Chilet, M., García-Casado, Z., Manrique-Silva, E., Requena, C., Bañuls, J., López-Guerrero, J.A., Rodríguez-Hernández, A., Traves, V., and Dopazo, J. (2021). Mutational Characterization of Cutaneous Melanoma Supports Divergent Pathways Model for Melanoma Development. Cancers, 13.
|
|