Characterization of a Syngeneic Orthotopic Model of Cholangiocarcinoma by [18F]FDG-PET/MRI-Reference-Cited by-同舟云学术

Characterization of a Syngeneic Orthotopic Model of Cholangiocarcinoma by [18F]FDG-PET/MRI

Published:2024-07-19 Issue:14 Volume:16 Page:2591
ISSN:2072-6694
Container-title:Cancers
language:en
Short-container-title:Cancers

Author:

Zachhuber Lena¹²,Filip Thomas³,Mozayani Behrang⁴,Löbsch Mathilde⁵,Scheiner Stefan⁶,Vician Petra⁶,Stanek Johann¹,Hacker Marcus²⁷^ORCID,Helbich Thomas H.¹⁸^ORCID,Wanek Thomas¹^ORCID,Berger Walter⁶^ORCID,Kuntner Claudia¹⁷^ORCID

Affiliation:

1. Preclinical Imaging Lab (PIL), Department of Biomedical Imaging and Image-Guided Therapy, Medical University of Vienna, 1090 Vienna, Austria

2. Division of Nuclear Medicine, Department of Biomedical Imaging and Image-Guided Therapy, Medical University of Vienna, 1090 Vienna, Austria

3. Institute of Animal Breeding and Genetics & Biomodels Austria, University of Veterinary Medicine, 1210 Vienna, Austria

4. Department of Pathology, Medical University of Vienna, 1090 Vienna, Austria

5. Core Facility Laboratory Animal Breeding and Husbandry, Medical University of Vienna, 1090 Vienna, Austria

6. Centre for Cancer Research and Comprehensive Cancer Center, Division of Applied and Experimental Oncology, Medical University of Vienna, 1090 Vienna, Austria

7. Medical Imaging Cluster (MIC), Medical University of Vienna, 1090 Vienna, Austria

8. Division of General and Pediatric Radiology, Department of Biomedical Imaging and Image-Guided Therapy, Medical University of Vienna, 1090 Vienna, Austria

Abstract

Cholangiocarcinoma (CCA) is a type of primary liver cancer originating from the biliary tract epithelium, characterized by limited treatment options for advanced cases and low survival rates. This study aimed to establish an orthotopic mouse model for CCA and monitor tumor growth using PET/MR imaging. Murine CCA cells were implanted into the liver lobe of male C57BL/6J mice. The imaging groups included contrast-enhanced (CE) MR, CE-MR with static [18F]FDG-PET, and dynamic [18F]FDG-PET. Tumor volume and FDG uptake were measured weekly over four weeks. Early tumor formation was visible in CE-MR images, with a gradual increase in volume over time. Dynamic FDG-PET revealed an increase in the metabolic glucose rate (MRGlu) over time. Blood analysis showed pathological changes in liver-related parameters. Lung metastases were observed in nearly all animals after four weeks. The study concludes that PET-MR imaging effectively monitors tumor progression in the CCA mouse model, providing insights into CCA development and potential treatment strategies.

Funder

Gesellschaft für Forschungsfoerderung Niederoesterreich m.b.H.

Publisher

MDPI AG

Link

https://www.mdpi.com/2072-6694/16/14/2591/pdf

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