DHX30 Coordinates Cytoplasmic Translation and Mitochondrial Function Contributing to Cancer Cell Survival

Author:

Bosco Bartolomeo,Rossi AnnalisaORCID,Rizzotto DarioORCID,Hamadou Meriem Hadjer,Bisio AlessandraORCID,Giorgetta Sebastiano,Perzolli AliciaORCID,Bonollo FrancescoORCID,Gaucherot Angeline,Catez FrédéricORCID,Diaz Jean-Jacques,Dassi ErikORCID,Inga AlbertoORCID

Abstract

DHX30 was recently implicated in the translation control of mRNAs involved in p53-dependent apoptosis. Here, we show that DHX30 exhibits a more general function by integrating the activities of its cytoplasmic isoform and of the more abundant mitochondrial one. The depletion of both DHX30 isoforms in HCT116 cells leads to constitutive changes in polysome-associated mRNAs, enhancing the translation of mRNAs coding for cytoplasmic ribosomal proteins while reducing the translational efficiency of the nuclear-encoded mitoribosome mRNAs. Furthermore, the depletion of both DHX30 isoforms leads to higher global translation but slower proliferation and lower mitochondrial energy metabolism. Isoform-specific silencing supports a role for cytoplasmic DHX30 in modulating global translation. The impact on translation and proliferation was confirmed in U2OS and MCF7 cells. Exploiting RIP, eCLIP, and gene expression data, we identified fourteen mitoribosome transcripts we propose as direct DHX30 targets that can be used to explore the prognostic value of this mechanism in cancer. We propose that DHX30 contributes to cell homeostasis by coordinating ribosome biogenesis, global translation, and mitochondrial metabolism. Targeting DHX30 could, thus, expose a vulnerability in cancer cells.

Funder

Institut National Du Cancer

Agence Nationale de la Recherche

Associazione Italiana per la Ricerca sul Cancro

Publisher

MDPI AG

Subject

Cancer Research,Oncology

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