Phenolic Profile, Inhibition of α-Amylase and α-Glucosidase Enzymes, and Antioxidant Properties of Solanum elaeagnifolium Cav. (Solanaceae): In Vitro and In Silico Investigations
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Published:2023-05-04
Issue:5
Volume:11
Page:1384
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ISSN:2227-9717
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Container-title:Processes
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language:en
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Short-container-title:Processes
Author:
Bouslamti Mohammed1, Loukili El Hassania2, Elrherabi Amal3, El Moussaoui Abdelfattah4ORCID, Chebaibi Mohamed5ORCID, Bencheikh Noureddine3ORCID, Nafidi Hiba-Allah6, Bin Jardan Yousef A.7ORCID, Bourhia Mohammed8ORCID, Bnouham Mohamed3, Lyoussi Badiaa1, Benjelloun Ahmed Samir1
Affiliation:
1. Laboratories of Natural Substances, Pharmacology, Environment, Modeling, Health and Quality of Life (SNAMOPEQ), Faculty of Sciences, Sidi Mohamed Ben Abdellah University, Fez 30000, Morocco 2. Laboratory of Applied Analytical Chemistry, Materials and Environment, Department of Chemistry, Faculty of Sciences, Mohammed First University, Oujda 717, Morocco 3. Laboratory of Bioresources, Biotechnology, Ethnopharmacology, and Health, Faculty of Sciences, Mohammed First University, Oujda 717, Morocco 4. Laboratory of Biotechnology, Environment, Agri-Food and Health, Faculty of Sciences Dhar El Mahraz, Sidi Mohammed Ben Abdellah University, Fez 30050, Morocco 5. Biomedical and Translational Research Laboratory, Faculty of Medicine and Pharmacy of the Fez, University of Sidi Mohamed Ben Abdellah, Fez 30070, Morocco 6. Department of Food Science, Faculty of Agricultural and Food Sciences, Laval University, 2325, Quebec City, QC G1V 0A6, Canada 7. Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia 8. Department of Chemistry and Biochemistry, Faculty of Medicine and Pharmacy, Ibn Zohr University, Laayoune 70000, Morocco
Abstract
In this study, the chemical composition and the antioxidant and antidiabetic properties of S. elaeagnifolium flower (SEFl), fruit (SEFr), and leaf (SEFe) extracts were investigated in vitro and in silico. HPLC-DAD analysis was used to determine the chemical components. Colorimetric techniques were used to identify polyphenols and flavonoids. The antioxidant capacity was determined using DPPH and TAC assays. The antidiabetic activity was examined using the enzymes α-amylase and α-glucosidase. Molecular docking methods were used to assess the anti-dipeptidyl peptidase IV (DPP-IV) activity. According to HPLC findings, extracts of S. elaeagnifolium flowers, leaves, and fruits are rich in salicylic acid, sinapic acid, chlorogenic acid, naringin, quercetin, quercetin-3-O-beta-glucoside, kaempferol, and chalcone. The IC50 for flower, leaf, and fruit extracts were 132 ± 5.59 μg/mL, 43.19 ± 1.46 μg/mL, and 132 ± 5.59 μg/mL, respectively. The total antioxidant capacity of SEFr, SEFe, and SEFl were determined to be 900.06 ± 4.01 μg AAE/mg, 792.10 ± 6.72 μg AAE/mg, and 681.10 ± 3.02 μg AAE/mg, respectively. Importantly, SEFe, SEFl, and SEFr displayed significant anti-α-amylase activity, with IC50 values of 79.16 ± 2.35 µg/mL, 99.16 ± 1.17 µg/mL, and 40.31 ± 2.04 µg/mL, respectively. The results also showed that SEFr, SEFe, and SEFl all exhibited potent anti-α-glucosidase activity, whose IC50 values were determined to be 20.53 ± 0.37 µg/mL (SEFr), 20.05 ± 0.12 µg/mL (SEFe), and 41.1 ± 1.55 µg/mL (SEFl). Molecular docking of S. elaeagnifolium phenolic compounds in the active site of DPP-IV revealed a strong inhibitory effect, with a glide score ranging from −2.63 to −8.10 Kcal/mol. Notably—with glide scores of −8.10, −6.23, −5.73, and −5.37 Kcal/mol—rutin, quercetin-3-O-beta-glucoside, chalcone, and naringin were the most active molecules against DPP-IV.
Funder
Researchers Supporting Project
Subject
Process Chemistry and Technology,Chemical Engineering (miscellaneous),Bioengineering
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