Luteolin Alleviates Cadmium-Induced Kidney Injury by Inhibiting Oxidative DNA Damage and Repairing Autophagic Flux Blockade in Chickens
-
Published:2024-04-26
Issue:5
Volume:13
Page:525
-
ISSN:2076-3921
-
Container-title:Antioxidants
-
language:en
-
Short-container-title:Antioxidants
Author:
Zhang Kanglei123, Li Jiahui123, Dong Wenxuan4, Huang Qing123, Wang Xueru123, Deng Kai123, Ali Waseem123, Song Ruilong123, Zou Hui123, Ran Di56, Liu Gang1237, Liu Zongping123ORCID
Affiliation:
1. College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China 2. Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China 3. Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou 225009, China 4. College of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266000, China 5. College of Veterinary Medicine, Southwest University, Chongqing 400715, China 6. College of Medicine, University of Illinois at Chicago, Chicago, IL 60607, USA 7. Department of Pathology and Laboratory Medicine, Tulane University School of Medicine, New Orleans, LA 70112, USA
Abstract
Chickens are a major source of meat and eggs in human food and have significant economic value. Cadmium (Cd) is a common environmental pollutant that can contaminate feed and drinking water, leading to kidney injury in livestock and poultry, primarily by inducing the generation of free radicals. It is necessary to develop potential medicines to prevent and treat Cd-induced nephrotoxicity in poultry. Luteolin (Lut) is a natural flavonoid compound mainly extracted from peanut shells and has a variety of biological functions to defend against oxidative damage. In this study, we aimed to demonstrate whether Lut can alleviate kidney injury under Cd exposure and elucidate the underlying molecular mechanisms. Renal histopathology and cell morphology were observed. The indicators of renal function, oxidative stress, DNA damage and repair, NAD+ content, SIRT1 activity, and autophagy were analyzed. In vitro data showed that Cd exposure increased ROS levels and induced oxidative DNA damage and repair, as indicated by increased 8-OHdG content, increased γ-H2AX protein expression, and the over-activation of the DNA repair enzyme PARP-1. Cd exposure decreased NAD+ content and SIRT1 activity and increased LC3 II, ATG5, and particularly p62 protein expression. In addition, Cd-induced oxidative DNA damage resulted in PARP-1 over-activation, reduced SIRT1 activity, and autophagic flux blockade, as evidenced by reactive oxygen species scavenger NAC application. The inhibition of PARP-1 activation with the pharmacological inhibitor PJ34 restored NAD+ content and SIRT1 activity. The activation of SIRT1 with the pharmacological activator RSV reversed Cd-induced autophagic flux blockade and cell injury. In vivo data demonstrated that Cd treatment caused the microstructural disruption of renal tissues, reduced creatinine, and urea nitrogen clearance, raised MDA content, and decreased the activities or contents of antioxidants (GSH, T-SOD, CAT, and T-AOC). Cd treatment caused oxidative DNA damage and PARP-1 activation, decreased NAD+ content, decreased SIRT1 activity, and impaired autophagic flux. Notably, the dietary Lut supplement observably alleviated these alterations in chicken kidney tissues induced by Cd. In conclusion, the dietary Lut supplement alleviated Cd-induced chicken kidney injury through its potent antioxidant properties by relieving the oxidative DNA damage-activated PARP-1-mediated reduction in SIRT1 activity and repairing autophagic flux blockade.
Funder
National Key R&D Program of China National Natural Science Foundation of China Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) and the Postdoctoral Research Funding of Yangzhou University
Reference77 articles.
1. Genchi, G., Sinicropi, M., Lauria, G., Carocci, A., and Catalano, A. (2020). The Effects of Cadmium Toxicity. Int. J. Environ. Res. Public Health, 17. 2. Current status, spatial features, health risks, and potential driving factors of soil heavy metal pollution in China at province level;Hu;Environ. Pollut.,2020 3. Current status of agricultural soil pollution by heavy metals in China: A meta-analysis;Huang;Sci. Total Environ.,2019 4. Cadmium, environmental exposure, and health outcomes;Satarug;Environ. Health Perspect.,2010 5. Historical perspectives on cadmium toxicology;Nordberg;Toxicol. Appl. Pharmacol.,2009
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
|
|