Potential Antidiabetic Activity of Apis mellifera Propolis Extraction Obtained with Ultrasound
Author:
Hernández-Martínez Javier A.1, Zepeda-Bastida Armando1ORCID, Morales-Rodríguez Irma1, Fernández-Luqueño Fabián2ORCID, Campos-Montiel Rafael1, Hereira-Pacheco Stephanie E.3ORCID, Medina-Pérez Gabriela1ORCID
Affiliation:
1. ICAP—Institute of Agricultural Sciences, Autonomous University of the State of Hidalgo, Tulancingo de Bravo 43000, Hidalgo, Mexico 2. Sustainability of Natural Resources and Energy Program, Cinvestav-Saltillo, Ramos Arizpe 25900, Coahuila, Mexico 3. Laboratorio de Interacciones Bióticas, Centro de Investigación en Ciencias Biológicas, Universidad Autónoma de Tlaxcala, Km 10.5 de la carretera San Martín Texmelucan, San Felipe Ixtacuixtla, Villa Mariano Matamoros 90120, Tlaxcala, Mexico
Abstract
Recent studies have linked phenolic compounds to the inhibition of digestive enzymes. Propolis extract is consumed or applied as a traditional treatment for some diseases. More than 500 chemical compounds have been identified in propolis composition worldwide. This research aimed to determine Mexican propolis extracts’ total phenolic content, total flavonoid content, antioxidant activity, and digestive enzyme inhibitory activity (ɑ-amylase and ɑ-glucosidase). In vitro assays measured the possible effect on bioactive compounds after digestion. Four samples of propolis from different regions of the state of Oaxaca (Mexico) were tested (Eloxochitlán (PE), Teotitlán (PT), San Pedro (PSP), and San Jerónimo (PSJ)). Ethanol extractions were performed using ultrasound. The extract with the highest phenolic content was PE with 15,362.4 ± 225 mg GAE/100 g. Regarding the flavonoid content, the highest amount was found in PT with 8084.6 ± 19 mg QE/100 g. ABTS•+ and DPPH• radicals were evaluated. The extract with the best inhibition concentration was PE with 33,307.1 ± 567 mg ET/100 g. After simulated digestion, phenolics, flavonoids, and antioxidant activity decreased by 96%. In contrast, antidiabetic activity, quantified as inhibition of ɑ-amylase and ɑ-glucosidase, showed a mean decrease in enzyme activity of approximately 50% after the intestinal phase. Therefore, it is concluded that propolis extracts could be a natural alternative for treating diabetes, and it would be necessary to develop a protective mechanism to incorporate them into foods.
Subject
Plant Science,Health Professions (miscellaneous),Health (social science),Microbiology,Food Science
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