An Analysis of Polysaccharides from Eight Plants by a Novel Heart-Cutting Two-Dimensional Liquid Chromatography Method

Author:

Wang Haonan12,Jin Hongyu1,Chai Ruiping3,Li Hailiang1,Fan Jing1,Wang Ying1ORCID,Wei Feng1ORCID,Ma Shuangcheng4

Affiliation:

1. National Institutes for Food and Drug Control, National Medical Products Administration, Beijing 102629, China

2. National Institutes for Food and Drug Control, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China

3. Thermo Fisher Scientific (China) Co., Ltd., Shanghai 201206, China

4. Chinese Pharmacopoeia Commission, Beijing 100061, China

Abstract

Natural polysaccharides are important active biomolecules. However, the analysis and structural characterization of polysaccharides are challenging tasks that often require multiple techniques and maps to reflect their structural features. This study aimed to propose a new heart-cutting two-dimensional liquid chromatography (2D–LC) method for separating and analyzing polysaccharides to explore the multidimensional information of polysaccharide structure in a single map. That is, the first-dimension liquid chromatography (1D–LC) presents molecular-weight information, and the second-dimension liquid chromatography (2D–LC) shows the fingerprints of polysaccharides. In this 2D–LC system, the size-exclusion chromatography–hydrophilic interaction chromatography (SEC–HILIC) model was established. Coupling with a charged aerosol detector (CAD) eliminated the need for the derivatization of the polysaccharide sample, allowing the whole process to be completed within 80 min. The methods were all validated in terms of precision, linearity, stability, and repeatability. The capability of the new 2D-LC method was demonstrated in determining various species of natural polysaccharides. Our experimental data demonstrated the feasibility of the whole systematic approach, opening the door for further applications in the field of natural polysaccharide analysis.

Funder

National Natural Science Foundation of China

NIFDC Fund for Key Technology Research

Publisher

MDPI AG

Reference44 articles.

1. Bioactivities, isolation and purification methods of polysaccharides from natural products: A review;Shi;Int. J. Biol. Macromol.,2016

2. Hepatoprotective effect of plant polysaccharides from natural resources: A review of the mechanisms and structure-activity relationship;Qu;Int. J. Biol. Macromol.,2020

3. Saccharide mapping and its application in quality control of polysaccharides from Chinese medicines;Li;Zhongguo Zhong Yao Za Zhi.,2015

4. Primary investigation on the quality control system in polysaccharide in traditional Chinese medicine;Wang;J. Pharm. Anal.,2021

5. A comparison study on digestion, anti-inflammatory and functional properties of polysaccharides from four Auricularia species;Li;Int. J. Biol. Macromol.,2020

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