Reciprocal Garden Study Reveals Acute Spatial-Edaphic Adaptation for Cycas micronesica

Abstract

A long-term reciprocal garden study was used to determine adaptive variation between Cycas micronesica K.D. Hill plants from north versus south Guam. Half-siblings from each location were planted as one-leaf seedlings in north and south gardens and monitored for 15 years. Stem height and diameter, and leaf number and maximum length were measured yearly. Survival and plant size traits were evaluated using a two-way factorial. In both locations, the local genotypes out-performed the foreign genotypes in terms of survival and growth. Survival of the foreign genotypes began to decline by year 4 and was less than 10% by year 15. Survival of the local genotypes was 70% for the north garden and 100% for the south garden. The north site was more hostile to plant performance because overall survival and plant growth were less than for the south site. The most likely environmental factor provoking local adaptation was highly contrasting soil characteristics between north and south Guam. The results indicates that long-term conservation success for C. micronesica and other cycad species must include the concept of local adaptation into decisions for transplantation and restoration projects.