Abstract
Rapid and accurate identification of Candida albicans from among other candida species is critical for cost-effective treatment and antifungal drug assays. Shape is a critical biomarker indicating cell type, cell cycle, and environmental conditions; however, most microfluidic techniques have been focused only on size-based particle/cell manipulation. This study demonstrates a sheathless shape-based separation of particles/cells using a viscoelastic non-Newtonian fluid. The size of C. albicans was measured at 37 °C depending on the incubation time (0 h, 1 h, and 2 h). The effects of flow rates on the flow patterns of candida cells with different shapes were examined. Finally, 2-h-incubated candida cells with germ tube formations (≥26 μm) were separated from spherical candida cells and shorter candida cells with a separation efficiency of 80.9% and a purity of 91.2% at 50 μL/min.
Subject
Electrical and Electronic Engineering,Mechanical Engineering,Control and Systems Engineering
Reference68 articles.
1. Guidelines for Treatment of Candidiasis
2. Serum tube identification of Candida albicans
3. Utility of the Germ Tube Test for Direct Identification of
Candida albicans
from Positive Blood Culture Bottles
4. Infectious Diseases and Medical Microbiology;Braude,1986
5. Germ Tube Formation in Candida Albicans: Evaluation of Human and Animal Sera and Incubation Atmosphere;JO;Shiraz E-Medical J.,2005
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