Comparative Transcriptome Analysis of Female and Male Fine-Patterned Puffer: Identification of Candidate Genes Associated with Growth and Sex Differentiation

Abstract

Sexual growth dimorphism is a common phenomenon in teleost fish. However, the mechanism of this complex phenomenon remains unclear. The fine-patterned puffer (Takifugu poecilonotus; Temminck and Schlegel, 1850) exhibits female-biased sexual size dimorphism similar to other pufferfish. In this study, the transcriptomes of female and male T.poecilonotus were sequenced, 285.95 million raw read pairs were generated from sequence libraries. After identification and assembly, a total of 149,814 nonredundant unigenes were obtained with an N50 length of 3538 bp. Of these candidates, 122,719 unigenes (81.91% of the total) were successfully annotated with multiple public databases. The comparison analysis revealed 10,385 unigenes (2034 in females and 8351 in males) were differentially expressed between different sexes of T.poecilonotus. Then, we identified many candidate growth- and sex-related genes, including Dmrt1, Sox3, Spatas, Prl/Prlr, fabps, Ghr, and Igf1r. In addition to these well-known genes, Fabp4 was identified for the first time in fish. Furthermore, 68,281 simple sequence repeats (SSRs) loci were screened and identified in the transcriptome sequence of T.poecilonotus. The results of our study could provide valuable information on growth- and sex-associated genes and facilitate further exploration of the molecular mechanism of sexual growth dimorphism.