Affiliation:
1. Institute of Pharmacology and Clinical Pharmacology, Faculty of Medicine, Comenius University Bratislava, Slovakia. andrea.gazova@fmed.uniba.sk
Abstract
Experimental data concerning the bioavailability of the different Mg-salts in human organism is inconsistent. Mg-absorption reported by clinical studies largely varies depending on the method used for evaluation. The aim of this study was to evaluate the bioavailability and accessibility of magnesium bound in different Mg-salt compounds, using an in vitro model of intestinal cell barrier. The study included a variety of inorganic (oxide, sulphate, chloride, carbonate) and organic salts (lactate, citrate, pidolate). Caco-2 cells were cultivated in a complete culture medium with different magnesium salts treatments in ascending concentrations. The viability and quantity of cells was analysed by FACS. Mg-absorption was analysed by a direct colorimetric assay, measured by spectrometry. T-test identified a significant decrease in cell count treatment with mg-lactate compared with citrate. Mg-pidolate showed a significantly higher cell viability compared with Mg-citrate, Mg-lactate and Mg-chloride. Even though the difference was not significant, we showed that an increase in Mg2+ salt concentration progressively decreased the cell count and the viability and the effect was universal for all the used Mg-salt treatments. Mg-citrate, chloride, and sulphate showed a significantly lower absorption compared to Mg-carbonate, pidolate and oxide. Our in vitro monolayer model of human intestinal transport showed that viability and quantity of cell decreased with increasing Mg-concentration. We admit that our experiment model may have some limitations in accurately describing an in vivo Mg2+ absorption. Moreover, it is also necessary to assess the relevance of our data in vivo and especially in clinical practice.
Publisher
Institute of Physiology of the Czech Academy of Sciences
Subject
General Medicine,Physiology
Reference33 articles.
1. BEHAR J: Magnesium absorption by the rat ileum and colon. Am J Physiol Leg 227: 334-340, 1974. https://doi.org/10.1152/ajplegacy.1974.227.2.334
2. BØHMER T, RØSETH A, HOLM H, WEBERG-TEIGEN S, WAHL L: Bioavailability of oral magnesium supplementation in female students evaluated from elimination of magnesium in 24-hour urine. Magnes Trace Elem 9: 272-278, 1990.
3. BRANNAN PG, VERGNE-MARINI P, PAK CY, HULL AR, FORDTRAN JS: Magnesium absorption in the human small intestine. Results in normal subjects, patients with chronic renal disease, and patients with absorptive hypercalciuria. J Clin Investig 57: 1412-1418, 1976. https://doi.org/10.1172/JCI108410
4. COUDRAY C, RAMBEAU M, FEILLET-COUDRAY C, GUEUX E, TRESSOL JC, MAZUR A, RAYSSIGUIER Y: Study of magnesium bioavailability from ten organic and inorganic Mg salts in Mg-depleted rats using a stable isotope approach. Magnesium Res 18: 215-223, 2005.
5. DE BAAIJ JHF, HOENDEROP JGJ, BINDELS RJM: Regulation of magnesium balance: Lessons learned from human genetic disease. Clin Kidney J 5: i15-i24, 2012. https://doi.org/10.1093/ndtplus/sfr164
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献