Author:
Štreitová D,Hofer M,Holá J,Vacek A,Pospíšil M
Abstract
Expression of mRNA for adenosine receptor subtypes A1, A2a, A2b,
and A3 in normal and lipopolysaccharide (LPS)-activated murine
RAW 264.7 macrophages has been investigated using the
method of quantitative real-time polymerase chain reaction. The
results have shown a very low, unquantifiable expression of
adenosine A1 receptor mRNA in both normal and LPS-activated
macrophages. The other three adenosine receptor mRNAs have
been found to be expressed at various but always quantifiable
levels. Activation of the macrophages by LPS induced
upregulation of the expression of adenosine receptor A2a and A2b
mRNA, whereas the expression of adenosine receptor A3 mRNA
was downregulated. Unstimulated macrophages exhibited a high
expression of the A2b adenosine receptor mRNA. The findings are
discussed from the point of view of the antiinflammatory and
hematopoiesis-stimulating roles of the adenosine receptor
signaling.
Publisher
Institute of Physiology of the Czech Academy of Sciences
Subject
General Medicine,Physiology
Cited by
15 articles.
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