Genistein does not Inhibit TGF-β1-Induced Conversion of Human Dermal Fibroblasts to Myofibroblasts-Reference-Cited by-同舟云学术

Genistein does not Inhibit TGF-β1-Induced Conversion of Human Dermal Fibroblasts to Myofibroblasts

Published:2021-10-31 Issue: Volume: Page:815-820
ISSN:1802-9973
Container-title:Physiological Research
language:en
Short-container-title:Physiol Res

Author:

KAŇUCHOVÁ M¹,URBAN L¹,MELEGOVÁ N¹,ČOMA M¹,DVOŘÁNKOVÁ B²,SMETANA K²,GÁL P¹

Affiliation:

1. Department of Pharmacology, Pavol Jozef Šafárik University, Košice, Slovak Republic

2. Institute of Anatomy, First Faculty of Medicine, Charles University, Prague, Czech Republic

Abstract

Transforming growth factor beta 1 (TGF-β1) is a pro-fibrotic cytokine with a key role in wound repair and regeneration, including induction of fibroblast-to-myofibroblast transition. Genistein is a naturally occurring selective estrogen receptor modulator with promising anti-fibrotic properties. In the present study we aimed to investigate whether genistein modulates TGF-β1 (canonical and non-canonical) signaling in normal dermal fibroblasts at the protein level (Western blot and immunofluo-rescence). We demonstrated that TGF-β1 induces the myofibroblast-like phenotype in the studied fibroblast signaling via canonical (SMAD) and non-canonical (AKT, ERK1/2, ROCK) pathways. Genistein induced only ERK1/2 expression, whereas the combination of TGF-β1 and genistein attenuated the ERK1/2 and ROCK signaling. Of note, the other studied pathways remained almost unaffected. From this point of view, genistein does not impair conversion of normal fibroblasts to myofibroblast-like cells.

Publisher

Institute of Physiology of the Czech Academy of Sciences

Subject

General Medicine,Physiology

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