Affiliation:
1. Laboratory of Transgenic Models of Diseases, Institute of Molecular Genetics, Czech Academy of Sciences, Prague, Czech Republic
Abstract
Lecithin:retinol acyltransferase (LRAT) is the major enzyme responsible for retinol esterification in the mammalian body. LRAT exhibits specific activity in the cells with active retinol metabolism where it converts retinols into retinyl esters, which represents the major storage form of retinol. Besides hepatic stellate cells in the liver, LRAT appears to have a key physiologic role in several other tissues. In this study, we generated a transgenic reporter mouse expressing green fluorescence protein (EGFP) under the control of region containing -1166 bps from promoter upstream from the putative transcriptional start site and 262 bps downstream of this start. Transgenic reporter mice exhibited specific expression in eyes and testes. In eyes, expression of EGFP-reporter is found in lens and lens epithelium and fibers from embryo to adulthood. In testes, LRAT-EGFP reporter is expressed both in Sertoli and in spermatocytes marking initiation of spermatogenesis in prepubertal mice. Our data show that the examined LRAT regulatory region is sufficient to achieve strong and selective expression in the eye and testes but not in liver and other organs.
Publisher
Institute of Physiology of the Czech Academy of Sciences
Subject
General Medicine,Physiology
Reference25 articles.
1. BATTEN ML, IMANISHI Y, MAEDA T, TU DC, MOISE AR, BRONSON D, POSSIN D, VAN GELDER RN, BAEHR W, PALCZEWSKI K: Lecithin-retinol acyltransferase is essential for accumulation of all-trans-retinyl esters in the eye and in the liver. J Biol Chem 279: 10422-10432, 2004.
2. BHAT SP: The ocular lens epithelium. Biosci Rep 21: 537-563, 2001.
3. D'AMBROSIO DN, CLUGSTON RD, BLANER WS: Vitamin A metabolism: an update. Nutrients 3: 63-103, 2011.
4. GUAN K, NAYERNIA K, MAIER LS, WAGNER S, DRESSEL R, LEE JH, NOLTE J, WOLF F, LI M, ENGEL W, HASENFUSS G: Pluripotency of spermatogonial stem cells from adult mouse testis. Nature 440: 1199-1203, 2006.
5. GUO X, KNUDSEN BS, PEEHL DM, RUIZ A, BOK D, RANDO RR, RHIM JS, NANUS DM, GUDAS LJ: Retinol metabolism and lecithin:retinol acyltransferase levels are reduced in cultured human prostate cancer cells and tissue specimens. Cancer Res 62: 1654-1661, 2002.
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献