GFP transgenic animals in biomedical research: a review of potential disadvantages-Reference-Cited by-同舟云学术

GFP transgenic animals in biomedical research: a review of potential disadvantages

Published:2019-08 Issue: Volume: Page:525-530
ISSN:1802-9973
Container-title:Physiological Research
language:en
Short-container-title:Physiol Res

Author:

Lipták N.¹,Bősze Z.,Hiripi L.

Affiliation:

1. NARIC-Agricultural Biotechnology Institute, Animal Biotechnology Department, Gödöllő, Hungary. E-mail: liptak.nandor@abc.naik.hu

Abstract

Green Fluorescent protein (GFP) transgenic animals are accepted tools for studying various physiological processes, including organ development and cell migration. However, several in vivo studies claimed that GFP may impair transgenic animals’ health. Glomerulosclerosis was observed in transgenic mice and rabbits with ubiquitous reporter protein expression. Heart-specific GFP expression evoked dilated cardiomyopathy and altered cardiac function in transgenic mouse and zebrafish lines, respectively. Moreover, growth retardation and increased axon swelling were observed in GFP and yellow fluorescent protein (YFP) transgenic mice, respectively. This review will focus on the potential drawbacks of the applications of GFP transgenic animals in biomedical research.

Publisher

Institute of Physiology of the Czech Academy of Sciences

Subject

General Medicine,Physiology

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