Myocardial m6A regulators in postnatal development: effect of sex-Reference-Cited by-同舟云学术

Myocardial m6A regulators in postnatal development: effect of sex

Published:2022-11-25 Issue:6 Volume:71 Page:877-882
ISSN:1802-9973
Container-title:Physiological Research
language:en
Short-container-title:Physiol Res

Author:

Semenovykh D,Benak D,Holzerova K,Cerna B,Telensky P,Vavrikova T,Kolar F,Neckar J,Hlavackova M¹

Affiliation:

1. Laboratory of Developmental Cardiology, Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic. E-mail: marketa.hlavackova@fgu.cas.cz

Abstract

N6-methyladenosine (m6A) is an abundant mRNA modification affecting mRNA stability and protein expression. It is a highly dynamic process, and its outcomes during postnatal heart development are poorly understood. Here we studied m6A machinery in the left ventricular myocardium of Fisher344 male and female rats (postnatal days one to ninety; P1-P90) using Western Blot. A downward pattern of target protein levels (demethylases FTO and ALKBH5, methyltransferase METTL3, reader YTHDF2) was revealed in male and female rat during postnatal development. On P1, the FTO protein level was significantly higher in male compared to females.

Publisher

Institute of Physiology of the Czech Academy of Sciences

Subject

General Medicine,Physiology

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