Affiliation:
1. Institute of Endocrinology, Prague, Czech Republic
Abstract
In order to assess whether intratesticular hormone content may be helpful for prediction of successful conception in men with fertility problems, five sex steroids, testosterone, dihydrotestosterone, androstenedione, estradiol and, for the first time epitestosterone, were measured in testicular tissue obtained by surgical retrieval from total 84 men. The group consisted of non-obstructive azoospermic men, aged 21-67 years who attended the centre for in vitro fertilization. Steroids after ether extraction and solvent partition were separated by high performance liquid chromatography and then measured by specific radioimmunoassays. The values varied considerably with means ± S.D. 2.43±2.47, 0.27±0.24, 0.080±0.13, 0.071±0.089 and 0.31±0.27 for testosterone, dihydrotestosterone, androstenedione, estradiol and epitestosterone, respectively.
Publisher
Institute of Physiology of the Czech Academy of Sciences
Subject
General Medicine,Physiology
Reference19 articles.
1. BAMBINO TH, HSUEH AJ: Direct inhibitory effect of glucocorticoids upon testicular luteinizing hormone receptor and steroidogenesis in vivo and in vitro. Endocrinology 108: 2142-2148, 1981.
2. BÍLEK R, HAMPL R, PUTZ Z, STÁRKA L: Radioimmunoassay of epitestosterone: methodology, thermodynamic aspects and applications. J Steroid Biochem 28: 723-729, 1987.
3. CARREAU S, BOURGUIBA S, MARIE E: Testicular and blood steroid levels in aged men. Reprod Biol 4: 299-304, 2004.
4. HAMPL R, PUTZ Z, STÁRKA L: Radioimmunological determination of dihydrotestosterone and its importance for laboratory diagnostics (in Czech). Biochem Clin Bohemoslov 19: 157-163, 1990.
5. JAROW JP, ZIRKIN BR: The androgen microenvironment of the human testis and hormonal control of spermatogenesis. Ann N Y Acad Sci 1061: 208-220, 2005.
Cited by
5 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献