STUDYING THE SOWS’ PLACENTAL TISSUE RECEPTIVITY WITH DIFFERENT ENZYMATIVE ACTIVITIES BASED ON IMMUNOHISTOCHEMICAL RESEARCH METHODS

Abstract

The purpose of research is to study the receptivity of placental tissue using immunohistochemical research methods. This group of diagnostic methods makes it possible to establish pathogenesis and pathomorphology before obvious clinical manifestations with the determination of markers that are an important element of the structure of the fetoplacental complex and its functioning. A change in the endothelial structures of the placenta was established in the form of expressive differences in the basal part of the chorionic villi and a defect in the syncytiocapillary membranes and endothelium of the villi capillaries. In the group of animals with high enzymatic activity of placental tissue, CD34 – immunopositive structures were detected in the paracentral zones of the chorionic villi in 87 % of individuals. This fact causes dystrophic processes in the walls of the placental vessels and indicates pathomorphological changes in the placental barrier. The expression of CD34 immunopositive cells in the endothelial vascular layer of the chorion was carried out by immunohistochemical study in a wiring system and incubation with antibodies according to the protocol from the manufacturer, with mouse monoclonal antibodies to CD34 – CD34 Mouse Monoclonal Antibody. The use of the proposed approaches for CD typing of the fetoplacental complex, depending on the enzymatic activity of the placenta, will make it possible to identify disorders and pathological conditions in order to prevent early reproductive losses in farm animals. Identification of characteristic conditions of placentation with a specific receptive profile makes it possible to objectively assess the immunogenic and reactive capabilities of the newborn organism, as well as to specify the tactics of subsequent pregnancies in productive animals.