FREE RADICAL GENERATION BY COW BLOOD CELLS AT SUBCLINICAL RADIATION DOSES

Author:

Fedotova Arina1,Turitsyna Evgeniya1,Makarskaya G2

Affiliation:

1. Krasnoyarskiy gosudarstvennyy agrarnyy universitet

2. FRC "Krasnoyarsk Scientific Center SB RAS"

Abstract

The aim of the study is to evaluate the kinetics of ROS generation in the peripheral blood of cattle exposed to subclinical doses of radiation. Objectives: to determine chemiluminescent indices: time to reach and amplitude of maximum activity, total ROS volume in the peripheral blood of lactating cows in agricultural landscapes of the Krasnoyarsk Region with different radioecological status; to calculate the blood phagocyte activation index. The object of the study is lactating cows kept in three agricultural landscapes of the Krasnoyarsk Region with different radioecological status: with an absorbed annual dose for farm animals of 0.92 mGy (control), with doses of 1.33 and 1.55 mGy/year. Research material is peripheral blood of cows. The studies were conducted in 2017–2019 in the immunology sector of the international scientific center for research into extreme states of the body, Federal Research Center of the Krasnoyarsk Scientific Center, Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk, and at the Institute of Applied Biotechnology and Veterinary Medicine, Federal State Budgetary Educational Institution of Higher Education, Krasnoyarsk State Agrarian University. In the peripheral blood of lactating cows, the following chemiluminescent indices were assessed: the time of formation of peaks in the production of reactive oxygen species and the amplitude of their generation, the total volume of ROS, and the index of activation of blood phagocytes. The chemiluminescent analysis was performed in the immunology sector of the international scientific center for research into extreme conditions of the body, FRC KSC SB RAS (Krasnoyarsk). Subclinical doses of ionizing radiation of 1.33 and 1.55 mGy/year negatively affect the course of free-radical processes in the blood cells of cows. Slowdown in the formation time of kinetic peaks, an increase in the total number of spontaneous free radicals, and a decrease in the light sum of activated ROS were revealed. A decrease in the cell activation index was established, i.e. a decrease in the ability of blood phagocytes to induce a respiratory burst in response to additional antigenic irritation, which is related to the negative consequences of exposure to low doses of ionizing radiation.

Publisher

Krasnoyarsk State Agrarian University

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