CD133 Progenitor Cells from the Bone Marrow Contribute to Retinal Pigment Epithelium Repair

Author:

Harris Jeffrey R.1,Fisher Robert1,Jorgensen Marda1,Kaushal Shalesh1,Scott Edward W.1

Affiliation:

1. Program in Stem Cell Biology, McKnight Brain Institute, University of Florida, Gainesville, Florida, USA

Abstract

Abstract Our goal was to define a clinically significant population of cells by utilizing a single-step selection process to enrich hematopoietic cells capable of regenerating the retinal pigment epithelium (RPE). Utilizing intravitreal injection of bone marrow cells from a mouse with pigment (C57BL6:gfp) into albino recipient mice (C57BL6:Tyr-), we show that hematopoietic progenitor cells (HPCs) enriched for CD133 can regenerate RPE cells and improve retinal function. The chemokine CXCL12 (stromal cell-derived factor 1α) is essential for migration, incorporation, and RPE regeneration by CD133+ HPCs. Once incorporated, CD133+ HPCs become pigmented, adopt an RPE morphology, and express RPE-specific proteins, leading to partial functional recovery by electroretinogram. Human CD133+ HPCs also incorporate in the retina and assume RPE morphology in nonobese diabetic/severe combined immunodeficient mice xenografts. These data show that a clinically accessible CD133+ hematopoietic cell can home to an injured RPE layer, differentiate into cells with significant RPE morphology, and provide therapeutic functional recovery of the visual cycle.

Funder

NIH

Publisher

Oxford University Press (OUP)

Subject

Cell Biology,Developmental Biology,Molecular Medicine

Reference43 articles.

1. Current concepts in the pathogenesis of age-related macular degeneration;Zarbin;Arch Ophthalmol,2004

2. Retinal and optic nerve diseases;Margalit;Artif Organs,2003

3. Complement factor H polymorphism in age-related macular degeneration;Klein;Science,2005

4. The retinal pigment epithelium: A versatile partner in vision;Bok;J Cell Sci Suppl,1993

5. Peroxidase diffusion in the normal and laser-coagulated primate retina;Peyman;Invest Ophthalmol,1972

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