Liriodendrin, ameliorates hypertension by calcium channel blockade and enhancing enos expression in wistar rats

Abstract

Introduction: Hypertension is found to be the prime cause ofdeath worldwide in spite of a number of available treatmentswhich suggests that there is a need of discovering new leadmolecules that would be more effective to treat cardiovasculardisease (CVD). Liriodendrin, the lignan phytoconstituent possessespotential pharmacological effects. Literature survey suggeststhat liriodendrin could be effective in mitigating hypertensionconsidering its structural similarity with reported cardiovascularprotective drugs. Hence liriodendrin is investigated to reveal itsmechanism of actions to support its antihypertensive property.Methods: Hypertension was induced in male wistar rats withDOCA salt. Hypertensive rats were treated with liriodendrin for4 weeks. Blood pressure, heart rate, body weight, lipid profile,serum nitrite levels, vascular reactivity to various catecholamines,in-vitro calcium channel blocking assays, antioxidantassay, determination of aortic calcium level, endothelial function,expression of eNOS analysis were studied.Result: Liriodendrin was found safe orally up to 2000 mg/kg. Itshowed a significant decrease in heart rate, blood pressure andmean arterial pressure. In-vitro study on the isolated rat aorta revealedthe calcium channel blocking potential of liriodendrin. Vascularreactivity to various catecholamines was normalized. Vascularendothelium was significantly protected by the enhancedrelease of nitric oxide and eNOS expression by the western blottechnique. Oxidative stress was also significantly reduced.Conclusion: Liriodendrin was found to be beneficial in hypertensionas it produced vasorelaxation by blocking calciumchannels, enhancing nitric oxide release, and reducing oxidativestress. Thus, liriodendrin may be useful to relieve hypertensionand cardiovascular complications.