Comparison of Antibacterial Activity of Ethyl Acetate Fractions Separated from Trichoderma with Commercial Antibiotic Methicillin Against Drug-Resistant Bacteria using Minimum Inhibitory Concentration Method

Abstract

Aim: To compare the antibacterial activity of ethyl acetate fractions of Trichoderma viride against Drug-resistant bacteria. Materials and method: The ethanolic extract was made from fully grown mycelia and Trichoderma was isolated from the soil sample. Four Methicillin-Resistant Staphylococcus aureus strains were examined with the ethyl acetate extract (MRSA). The sample size was calculated using Clincalc.com, which revealed that the alpha error-threshold 0.05, the confidence interval 95%, the g-power 80%, and the enrollment ratio was 1. Groups 1 and 2 with sample size 12 each which represented drug-resistant bacteria and ethyl acetate fractions respectively. Result: The statistical Independent Sample T-Test analysis showed significant antibacterial activity against MRSA for the Trichoderma ethanolic extract with the statistical significance (p<0.05) when compared to the Susceptible bacterial strains. Conclusion: The tested ethyl acetate extract showed the MRSA at 100 μg/ml.