Comparative Studies on the Effect of Tris-Citric AcidFructose Egg Yolk Glycerol and Tris-Egg Yolk-Glucose based Semen Extender on various Semen Parameters for Effective Long Term Cryopreservation

Abstract

Aim: To evaluate the suitable Semen extender for a long duration of cryopreservation among Tris citric acid fructose egg yolk glycerol extender and Tris-egg yolk-glucose extender,. Materials and Methods: The sample size has been calculated by the clinCalc with an alpha error-threshold at 0.05, 95 % confidence interval (CI), G power at 80%, and enrolment ratio 1. Our study has been repeated 14 times which is (N=14) and it was measured using statistical Independent Samples Test. The study involved 28 sample sizes with 2 different groups with each group containing 14 samples. The collected semen samples are diluted with the Tris-citric acid-fructose egg yolk glycerol extender and Tris-egg yolk-glucose extender, based novel semen extenders. A small quantity of the diluted semen sample was taken to a counting device known as makler counting chamber used for analyzing the sperm count and motility of the semen samples. Results: The Tris-citric acid-fructose egg yolk glycerol extenders were better than Tris-egg yolk-glucose extender. So the estimated sperm count and sperm motility were found to be P <0.05, revealing the statistical insignificance. The characteristics after cryopreservation were decreased than the characteristics before cryopreservation. Conclusion: The sperm parameters were preserved well in Tris-citric acid-fructose egg yolk glycerol semen extender when compared with Tris-egg yolk-glucose based semen extender with respect to sperm count and sperm motility.