A Western Diet Increases Serotonin Availability in Rat Small Intestine

Author:

Bertrand R. L.1,Senadheera S.12,Markus I.2,Liu L.2,Howitt L.2,Chen H.2,Murphy T. V.1,Sandow S. L.2,Bertrand P. P.1

Affiliation:

1. Departments of Physiology (R.L.B., S.S., T.V.M., P.P.B.), School of Medical Sciences, University of New South Wales, Sydney NSW 2052 Australia

2. Pharmacology (S.S., I.M., L.L., L.H, H.C., S.L.S.), School of Medical Sciences, University of New South Wales, Sydney NSW 2052 Australia

Abstract

Abstract Diet-induced obesity is associated with changes in gastrointestinal function and induction of a mild inflammatory state. Serotonin (5-HT) containing enterochromaffin (EC) cells within the intestine respond to nutrients and are altered by inflammation. Thus, our aim was to characterize the uptake and release of 5-HT from EC cells of the rat ileum in a physiologically relevant model of diet-induced obesity. In chow-fed (CF) and Western diet–fed (WD) rats electrochemical methods were used to measure compression evoked (peak) and steady state (SS) 5-HT levels with fluoxetine used to block the serotonin reuptake transporter (SERT). The levels of mRNA for tryptophan hydroxylase 1 (TPH1) and SERT were determined by quantitative PCR, while EC cell numbers were determined immunohistochemically. In WD rats, the levels of 5-HT were significantly increased (SS: 19.2±3.7 μm; peak: 73.5±14.1 μm) compared with CF rats (SS: 12.3±1.8 μm; peak: 32.2±7.2 μm), while SERT-dependent uptake of 5-HT was reduced (peak WD: 108% of control versus peak CF: 212% control). In WD rats, there was a significant increase in TPH1 mRNA, a decrease in SERT mRNA and protein, and an increase in EC cells. In conclusion, our data show that foods typical of a Western diet are associated with an increased 5-HT availability in the rat ileum. Increased 5-HT availability is driven by the up-regulation of 5-HT synthesis genes, decreased re-uptake of 5-HT, and increased numbers and/or 5-HT content of EC cells which are likely to cause altered intestinal motility and sensation in vivo.

Publisher

The Endocrine Society

Subject

Endocrinology

Reference66 articles.

1. Neuroanatomy of extrinsic afferents supplying the gastrointestinal tract;Berthoud;Neurogastroenterol Motil,2004

2. Serotonin release and uptake in the gastrointestinal tract.;Bertrand;Auton Neurosci,2010

3. Sensory transmission in the gastrointestinal tract.;Blackshaw;Neurogastroenterol Motil,2007

4. Molecular defects in mucosal serotonin content and decreased serotonin reuptake transporter in ulcerative colitis and irritable bowel syndrome.;Coates;Gastroenterology,2004

5. Analysis of real-time serotonin (5-HT) availability during experimental colitis in mouse;Bertrand;Am J Physiol,2010

Cited by 89 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3