Affiliation:
1. Instituto Potosino de Investigación Científica y Tecnológica, Molecular Biology Division, San Luis Potosí 78216, México
Abstract
Abstract
Isoorientin (ISO) is a plant C-glycosylflavonoid with purported antidiabetic effects but unexplored mechanisms of action. To gain insight into its antidiabetic mechanisms, we assayed nontoxic ISO concentrations on the 2-(N-(7-nitrobenz-2-oxa-1, 3-diazol-4-yl) amino)-2-deoxy-d-glucose (2-NBDG) uptake by murine 3T3-F442A and human sc adipocytes. In insulin-sensitive adipocytes, ISO stimulated the 2-NBDG uptake by 210% (murine) and 67% (human), compared with insulin treatment. Notably, ISO also induced 2-NBDG uptake in murine (139%) and human (60%) adipocytes made resistant to insulin by treatment with TNF-α, compared with the incorporation induced in these cells by rosiglitazone. ISO induction of glucose uptake in adipocytes was abolished by inhibitors of the insulin signaling pathway. These inhibitors also blocked the proper phosphorylation of insulin signaling pathway components induced by ISO in both insulin-sensitive and insulin-resistant adipocytes. Additionally, ISO stimulated the transcription of genes encoding components of insulin signaling pathway in murine insulin-sensitive and insulin-resistant adipocytes. In summary, we show here that ISO exerts its antidiabetic effects by activating the insulin signaling pathway in adipocytes, reverts the insulin resistance caused in these cells by TNF-α by stimulating the proper phosphorylation of proteins in this signaling pathway, and induces the expression of genes encoding these proteins.
Reference43 articles.
1. Classification of diabetes mellitus: criteria for diagnosis;Serrano-Rios,2010
2. Diagnosis and classification of diabetes mellitus.;American Diabetes Association;Diabetes Care,2012
3. The general epidemiology of type 2 diabetes mellitus. New insights in prediabetes;Zimmet,2010
4. Regulated membrane trafficking of the insulin-responsive glucose transporter 4 in adipocytes.;Watson;Endocr Rev,2004
5. Mécanismes de la prise de poids en cas de normalisation glycémique.;Laville;Diabetes Metab,2000
Cited by
40 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献