Author:
Apaja Pirjo M.,Aatsinki Jyrki T.,Rajaniemi Hannu J.,Petäjä-Repo Ulla E.
Abstract
AbstractThe LH receptor (LHR) is a G protein-coupled receptor involved in the regulation of ovarian and testicular functions. In this study we demonstrate novel and unexpected patterns of receptor expression and regulation in fetal and adult rodent urogenital and adrenal tissues. Two rat LHR promoter fragments (∼2 and 4 kb) were shown to direct expression of the lacZ reporter in transgenic mice to gonads, adrenal glands, and kidneys, starting at 14.5 d post coitum, and to genital tubercles, starting at 11.5 d post coitum. These tissues were also found to express the full-length LHR mRNA and protein during rat fetal development, but, importantly, only immature receptors carrying unprocessed N-linked glycans were detected. After birth, the receptor gene activity ceased, except in the gonads, which started to express the mature receptor carrying fully processed N-linked glycans. Surprisingly, both LHR mRNA and mature protein levels were up-regulated substantially in pregnant female adrenal glands and kidneys at a time that coincides with differentiation of fetal urogenital tissues. Taken together, these results indicate that the LHR protein is expressed constitutively in gonadal and nongonadal urogenital tissues as well in adrenal glands, but its final functional maturation at the posttranslational level appears to be developmentally and physiologically regulated.
Reference51 articles.
1. The lutropin/choriogonadotropin receptor, a 2002 perspective;Ascoli;Endocr Rev,2002
2. The presence of gonadotropin receptors in nonpregnant human uterus, human placenta, fetal membranes, and decidua;Reshef;J Clin Endocrinol Metab,1990
3. Expression of the LH/CG receptor gene in various rat tissues;Pietilä;J Physiol Pharmacol,1996
4. Expression and localization of luteinizing hormone receptor in the female mouse reproductive tract;Zhang;Biol Reprod,2001
5. Novel expression of functional human chorionic gonadotropin/luteinizing hormone receptor gene in human umbilical cords;Rao;J Clin Endocrinol Metab,1993
Cited by
37 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献