Autocrine Positive Feedback Regulation of Prolactin Release From Tilapia Prolactin Cells and Its Modulation by Extracellular Osmolality

Author:

Yamaguchi Yoko1,Moriyama Shunsuke2,Lerner Darren T.3,Grau E. Gordon1,Seale Andre P.14

Affiliation:

1. Hawai'i Institute of Marine Biology (Y.Y., D.T.L., E.G.G., A.P.S.), University of Hawai'i at Mānoa, Kāne'ohe, Hawai'i 96744;

2. School of Marine Biosciences (S.M.), Kitasato University, Minami-ku, Sagamihara, Kanagawa 252-0373, Japan;

3. University of Hawai'i Sea Grant College Program (D.T.L.), University of Hawai'i at Mānoa, Honolulu, Hawai'i 96822

4. Department of Human Nutrition, Food and Animal Sciences (A.P.S.), University of Hawai'i at Mānoa, Honolulu, Hawai'i 96822

Abstract

Prolactin (PRL) is a vertebrate hormone with diverse actions in osmoregulation, metabolism, reproduction, and in growth and development. Osmoregulation is fundamental to maintaining the functional structure of the macromolecules that conduct the business of life. In teleost fish, PRL plays a critical role in osmoregulation in fresh water. Appropriately, PRL cells of the tilapia are directly osmosensitive, with PRL secretion increasing as extracellular osmolality falls. Using a model system that employs dispersed PRL cells from the euryhaline teleost fish, Oreochromis mossambicus, we investigated the autocrine regulation of PRL cell function. Unknown was whether these PRL cells might also be sensitive to autocrine feedback and whether possible autocrine regulation might interact with the well-established regulation by physiologically relevant changes in extracellular osmolality. In the cell-perfusion system, ovine PRL and two isoforms of tilapia PRL (tPRL), tPRL177 and tPRL188, stimulated the release of tPRLs from the dispersed PRL cells. These effects were significant within 5–10 minutes and lasted the entire course of exposure, ceasing within 5–10 minutes of removal of tested PRLs from the perifusion medium. The magnitude of response varied between tPRL177 and tPRL188 and was modulated by extracellular osmolality. On the other hand, the gene expression of tPRLs was mainly unchanged or suppressed by static incubations of PRL cells with added PRLs. By demonstrating the regulatory complexity driven by positive autocrine feedback and its interaction with osmotic stimuli, these findings expand upon the knowledge that pituitary PRL cells are regulated complexly through multiple factors and interactions.

Publisher

The Endocrine Society

Subject

Endocrinology

Reference82 articles.

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