INVESTIGATION OF THIOL-DISULFITE HOMEOSTASIS IN BLOOD TRANSFUSIONS IN EMERGENCY DEPARTMENT

Abstract

Aim: This study aims to evaluate thiol-disulfide homeostasis, which is one of the oxidative stress factors, in patients who have undergone transfusion of different amounts and different blood products in the emergency department, and to investigate whether it can be used as a marker for oxidative stress level. Material and methods: Patients over 18 years of age who were symptomatic and had a hemoglobin value of < 7g/dl and/or hematocrit < 21% were included in the study. Blood samples were taken from the patients included in the study before and 1 hour after the transfusion into a yellow capped biochemistry tube to study the thiol-disulfide homeostasis level. In addition, the patients' age, height, weight, pre-transfusion laboratory values, which blood product was transfused, and the amount of transfusion were recorded in the case forms we prepared, and a comparison was made between these collected data. The patients in the study group were grouped according to their existing chronic diseases as those without a known chronic disease, with one chronic disease and multiple chronic diseases. Results: When the values of native thiol, total thiol, and disulfide before and after the transfusion were compared, although there was an increase in the values, the p values were calculated as 0,124, 0,103, 0,247, respectively, and no statistically significance was found. After grouping according to the presence of chronic disease, no significant changes were found in the thiol-disulfide parameters of the patients before and after transfusion. Similarly, when the differences in the number of transfusion units administered were compared, no significant data could be reached. Conclusion: Thiol - disulfide homeostasis in the patient group included in the study was not found to be significant in the oxidative stress evaluation performed before and at the 1st hour after transfusion. We believe that the effectiveness of thiol-disulfide homeostasis, which is a biochemical parameter that is easy, cheap, and fast to evaluate, should be investigated in larger-scale studies.