Affiliation:
1. Federal Scientific Center for Medical and Preventive Health Risk Management Technologies
Abstract
Introduction. The impact of chemical and biological environmental factors is associated with the risk of a genetic predisposition to the development of cardiovascular and cancer-associated diseases, which determines the relevance of the search for genetic indicator markers of early disorders in the mRNA structure.
Materials and methods. The analysis of TP53 rs1042522 gene polymorphism, as well as the relative normalized expression level of TP53 hs1034249_m1 transcript, in whole blood cell culture in healthy volunteers, both spontaneous and induced by 24-hour incubation with benzo[a]pyrene and SARS-CoV-2 vaccine antigen
(at concentrations of 0.006 mg/kg). Ml), was conducted.
Results. Comparative analysis of spontaneous and antigen-induced levels of TP53 hs1034249_m1 mRNA expression allowed establishing individual and group values of relative expression associated with the polymorphism features of the TP53 rs1042522 gene. Benzo[a]pyrene and SARS-CoV-2 were found to have opposite effects on the expression of hs1034249_m1 TP53 genes in the case of the CG rs1042522 genotype, while the combined effect of benzo[a]pyrene and SARS-CoV-2, which reflected the inhibition of the expression of hs00900055_m1 of the TP53 gene was associated with the GG genotype.
The limitations of the study are the use of a relatively small sample and a limited number of whole blood samples.
Conclusion. The ability of benzo[a]pyrene and SARS-CoV-2 at concentrations of 6 µg/L to modify the expression of the TP53 apoptosis gene in vitro has been shown, which makes it possible to consider the increase in the expression of hs00900055_m1 of the TP53 gene induced by benzo[a]pyrene as one of the mechanisms for aggravating the course of viral infections (SARS-CoV-2) in connection with loss of p53-controlling for the development of inflammation (its proliferative phase) for owners of the heterozygous variant of CG TP53 rs1042522. In the case of variant monozygotic polymorphism GG TP53 rs1042522, the combination of benzo[a]pyrene and SARS-CoV-2 leads to inhibition of the expression of hs00900055_m1 mRNA of the TP53 gene, which is phenotypically reflected by the formation of asthenia, immunosuppression and onco-proliferative complications. The hs00900055_m1 transcript of the TP53 gene is recommended as an indicator for the tasks of diagnosing early disorders associated with the combination of SARS-CoV-2+ benzo[a]pyrene. The experiment simulates the natural conditions of real combinations of influencing factors.
Publisher
Federal Scientific Center for Hygiene F.F.Erisman
Subject
Health, Toxicology and Mutagenesis,Public Health, Environmental and Occupational Health,Pollution,General Medicine
Reference19 articles.
1. Zaytseva N.V., Dolgikh O.V., Dianova D.G. Haptens of Natural and Man-Made Origin and Cell Death [Gapteny prirodnogo i tekhnogennogo proiskhozhdeniya i kletochnaya gibel’]. Perm’; 2020. https://elibrary.ru/mltypp (in Russian)
2. Makarov V.Z., Gusev V.A., Volkov Yu.V., Zatonskiy V.A., Nevryuev A.M. Benzopyrene in the atmosphere of Saratov region cities. Izvestiya Saratovskogo universiteta. Novaya seriya. Seriya: Nauki o Zemle. 2019; 19(1): 12–7. https://doi.org/10.18500/1819-7663-2019-19-1-12-17 https://elibrary.ru/zadhrz
3. Shelepova V.S., Zvyagintseva A.V. Benzapyrene – chemical and biological problem of modernity (C20H12). Pozharnaya bezopasnost’: problemy i perspektivy. 2017; 1(8): 477–80. https://elibrary.ru/zpejix
4. Chelakova Yu.A., Kazakova O.A., Dolgikh O.V. Analysis of cell death indicators by employees under production exposure to phenol. Rossiyskiy immunologicheskiy zhurnal. 2018; 12(4): 779–81. https://doi.org/10.31857/S102872210002673-5 https://elibrary.ru/vrwjgt
5. Zaytseva N.V., Ulanova T.S., Dolgikh O.V., Nurislamova T.V. Association analysis-based study results in evaluating the aromatic hydrocarbons contamination level of bio media and immunotropic effects of oil and gas enterprises employees from various work experience groups. Yakutskiy meditsinskiy zhurnal. 2020; (3): 25–8. https://doi.org/10.25789/YMJ.2020.71.06 https://elibrary.ru/daaycg