Cytomic analysis: a modern universal tool for biomedical and ecological and hygienic research (literature review). Part 2

Abstract

In the second part of the review, examples of cytome analysis on both peripheral blood lymphocytes and human buccal epiteliocytes are given in the study of occupational risks and investigation of genome instabilities induced by alcohol and tobacco use. Much attention is paid to the cytome analysis application in environmental and hygienic research and the studies directed to evaluating natural and anthropogenic load to the human population, especially industrial, factors. The method of cytome analysis on primary culture of human peripheral blood lymphocytes cultivated with a cytokinetic block (CBMN-cyt test) in its current state is described in detail, and the history of its development is briefly reflected. The method of cytome analysis on human buccal epiteliocytes (BMN-cyt) is also analyzed. The review contains photomicrographs of genetic cell damages that are markers in micronuclei (MN) tests. We used PubMed, Web of Science, ResearchGate, Scopus, eLibrary databases as the sources of literature.