CHANGES IN LIPID PEROXIDATION IN THE REPRODUCTIVE ORGANS OF MALE RATS DUE TO THE TRANSPLACENTAL ACTION OF CHEMICAL ENDOCRINE DISRUPTORS

Abstract

Abstract. In everyday life, people come into contact with chemical products that have hormone-like properties, for example, antiandrogenic (dibutyl phthalate, DBP) or estrogenic (bisphenol A, BPA). For a number of years, the issue of the potential harm of low doses of these endocrine disruptors (ED) for a developing fetus after entering the body of a pregnant mother has been discussed. Taking into consideration the ED ability to overcome the placental barrier, and the fact that one of the general mechanisms of the pathogenic effect of ED on humans and animals is oxidative stress, it is reasonable to study the state of lipid peroxidation (LPO) in the reproductive organs of adult male offspring, which was subjected to prenatal effects of low doses of DBP and BPA. The aim is to elucidate the prenatal effect of low doses of DBP and BPA on the content of LPO products in the ventral prostate (VP) and gonads of adult male rats. Material and methods. Wistar rats received orally an oil solution of DBP at 100 mg / kg bw. or BPA in Dorfman gel at 25 μg / kg bw per day from 15 to 21 days of pregnancy, control animals – carriers of drugs. In male offspring of 6 and 18 months of age, the contents of LPO products – malonic dialdehyde (MDA) and diene conjugates (DC) – were determined per mg of protein in the VP and testes. Results. In the testes of young rats prenatally exposed to DBP, the contents of both studied LPO products were significantly increased. The content of MDA in the VP of aging experimental rats exceeded 6 times, and DC – 1.5 times the values of control animals. The expression levels of MDA and DC in the testes of experimental animals increased by 134 % and 37 %, respectively. In the testes of 6-month-old rats exposed to BPA in utero, the contents of LPO products significantly increased in comparison with the control group: MDA by 155 %, DC – by 16 %. In the VP, the content of MDA significantly increased, DC did not differ from the control group. Conclusions. The result of oral administration of DBP to rats at a threshold dose relative to reproductive fetotoxicity during the last week of pregnancy is the activation of LPO in the testes of young and in the VP and testes of aging offspring. Oral administration of a subthreshold BPA dose to rats in the last week of pregnancy leads to the activation of LPO in the testes and the VP of young offspring. Key words: dibutyl phthalate, bisphenol A, prenatal action, lipid peroxidation, male rats, ventral prostate, testes.